Showing 1 – 9 of 9
Relevance | Newest | Oldest |
  • In Vitro Propagation of Human Prepubertal Spermatogonial Stem Cells

    Abstract Full Text
    free access
    JAMA. 2011; 305(23):2416-2418. doi: 10.1001/jama.2011.791
  • JAMA June 15, 2011

    Figure 2: Detection of Human Prepubertal Spermatogonial Stem Cells After Transplantation to Immunodeficient Mouse Testis

    Migration of human spermatogonial stem cells (cultured cells from testicular cell culture of the 8-year-old boy, passage 6 at 9 weeks) to the basal membrane of the seminiferous epithelium of immunodeficient mouse testis 8 weeks after transplantation. Cells were detected using a (A) human COT-1 fluorescence in situ hybridization probe, and detection with Cy3 (red) and cells were visualized using (B) DAPI (blue) staining. The merged image (C) indicates COT-1 staining in the nucleus of a migrated human spermatogonial stem cell.
  • Propagation of Human Spermatogonial Stem Cells In Vitro

    Abstract Full Text
    free access
    JAMA. 2009; 302(19):2127-2134. doi: 10.1001/jama.2009.1689
  • JAMA November 18, 2009

    Figure 4: PLZF Expression in Human Testis and Cultured Testicular Cells

    A, PLZF (promyelocytic leukemia zinc finger protein) immunofluorescence of Cy3 (red) staining on subcultured germline stem cells from study participant URO0021 (cluster 4, passage 4) with 4′,6-diamidino-2-phenylindol (DAPI) (blue) as a nuclear stain. B, Specific immunohistochemical (3,3′-diaminobenzidine [DAB]) localization of PLZF (brown) in the nuclei of type A spermatogonia in human testicular section (study participant UMC0001) (counterstained with hematoxylin [blue]).
  • JAMA November 18, 2009

    Figure 5: Detection of Human Spermatogonial Cells After Stem Cell Transplantation to Immunodeficient Mouse Testis

    Detection of human spermatogonial stem cells after transplantation to immunodeficient mouse testis using human COT-1 fluorescence in situ hybridization (FISH) Cy3 (red, left panel), nuclear counterstaining with 4′,6-diamidino-2-phenylindol (DAPI) (blue, middle panel) and merged images of COT-1 and DAPI (right panel). A, Adult testis control. COT-1 DNA FISH nuclear signal is present in all cells of adult human testis. B, Immunodeficient mouse testis 2 hours after transplantation. COT-1 FISH–positive human cells are detected in the lumen of the mouse seminiferous tubules. C, Immunodeficient mouse testis 10 weeks after transplantation. Homing of human spermatogonial stem cell from long-term subcultured germline stem cells to the basal membrane of the seminiferous epithelium of mouse testis. Insets are a higher magnification of the colonized human spermatogonial stem cell, indicated by arrowheads.
  • Search for male contraceptive complicated by adverse effects

    Abstract Full Text
    JAMA. 1984; 252(9):1101-1102. doi: 10.1001/jama.1984.03350090001001
  • A METHOD FOR THE PREDICTION OF SEX IN THE UNBORN: A PRELIMINARY REPORT

    Abstract Full Text
    JAMA. 1932; 99(20):1659-1660. doi: 10.1001/jama.1932.02740720013004
  • PHYSIOLOGIC EFFECTS OF SPERMATOXIN: IN RATS, RABBITS AND GUINEA-PIGS

    Abstract Full Text
    JAMA. 1926; 86(23):1755-1758. doi: 10.1001/jama.1926.02670490017006
  • THE REACTION OF ABDOMINAL TUMORS TO RADIATION

    Abstract Full Text
    JAMA. 1924; 83(2):109-113. doi: 10.1001/jama.1924.02660020031012