TY  - JOUR
T1  - SUperiority of the method of ice-box fixation in the wassermann test
AU  - Berghausen O
Y1  - 1919/04/05
N1  - 10.1001/jama.1919.26110140004011c
JO  - Journal of the American Medical Association
SP  - 996
EP  - 996
VL  - 72
IS  - 14
N2  - In 1917, Smith and MacNeal reported results obtained in a comparative study of different antigens and of different temperatures of incubation in the Wassermann test. Last summer the method of ice-box fixation was employed at the Cincinnati General Hospital with such gratifying results that it is now employed as a routine. Previously the classical test in which three different antigens were used, a double unit of complement and amboceptor, in a final volume of 2.5 c.c., with incubation at 37 C. in a water bath, was employed. Comparative studies have convinced us that when small amounts of antigen are used with fixation at the low temperatures of 0 and 2 C., false positive reactions are avoided and reliable results are obtained.PREPARATION OF ANTIGENS AND THE TECHNIC OF THE TEST 
    The cholesterinized antigen was prepared by treating 100 gm. of beef heart with 900 c.c. of alcohol in the usual
SN  - 0002-9955
M3  - doi: 10.1001/jama.1919.26110140004011c
UR  - http://dx.doi.org/10.1001/jama.1919.26110140004011c
ER  -