RT Journal
T1 CUltivation of the spirochæta pallida
JF Journal of the American Medical Association
JO Journal of the American Medical Association
YR 1909
FD September 11
VO LIII
IS 11
SP 874
OP 875
DO 10.1001/jama.1909.02550110048008
UL http://dx.doi.org/10.1001/jama.1909.02550110048008
AB Since Schaudinn several years ago discovered the Spirochæta pallida, many attempts have been made to grow the organism on artificial media, but only recently has any degree of success been obtained. Schereschewsky1 observed multiplication of the spirochete in pure horse serum. This medium heated to 58 or 60° C. for an hour or more becomes gelatinous, though it remains perfectly clear. Material obtained from a syphilitic papule or condyloma is immersed in this fluid and placed under strict anaerobic conditions in the incubator. After a number of days the culture is examined and by both the staining method and dark-ground illumination a notable increase in the number of organisms is seen. He recommends the dark-ground illuminator as very satisfactory and easily recognizes the Spirochæta pallida by this method. In a more recent paper2 he gives the results of more extensive studies. In the first culture shortly after inoculation