RT Journal
A1 Ehrenreich T, Kerpe S
T1 NEw, rapid method of obtaining dry, fixed cytological smears
JF Journal of the American Medical Association
JO Journal of the American Medical Association
YR 1959
FD July 4
VO 170
IS 10
SP 1176
OP 1177
DO 10.1001/jama.1959.63010100002012a
UL http://dx.doi.org/10.1001/jama.1959.63010100002012a
AB Since Papanicolaou1 first described a procedure of preparing smears for cytological examination numerous investigators have attempted to improve the method of fixation. Their principal purpose was to achieve good preservation of cells with a minimum time spent in fixing the slides and a convenient and safe means of transporting these slides to the laboratory.The original method consisted of placing freshly prepared smears into a bottle containing a mixture of equal parts of alcohol and ether. The cellular fixation resulting from this method is excellent, and it is still being widely used even though the bottles are bulky, subject to breakage, and not suitable for mailing. Several workers have made innovations principally in order to render the slides amenable to transportation in a dry state.Ayre and Dakin2 suggested fixing slides in alcohol-ether and then covering them with glycerine and another clean slide. Sagi and Mackenzie3 described