RT Journal
A1 Hall IC
T1 SElective elimination of hay bacillus from cultures of obligative anaerobes
JF Journal of the American Medical Association
JO Journal of the American Medical Association
YR 1919
FD January 25
VO 72
IS 4
SP 274
OP 275
DO 10.1001/jama.1919.26110040001016
UL http://dx.doi.org/10.1001/jama.1919.26110040001016
AB Repeated plating and streaking out on agar plates are universally acknowledged to be fairly reliable procedures for the isolation of aerobic bacteria irrespective of the possible concurrence of obligative anaerobes, for the latter, if present, are completely eliminated by the conditions of aerobic culture. But the same procedures applied under anaerobic conditions for the isolation of obligative anaerobes from cultures mixed with aerobes involve much uncertainty owing to the fact that many aerobes are also facultative anaerobes. It is therefore necessary to eliminate all aerobes from the culture before attempting the purification of obligative anaerobes by any method of surface colony isolation. It is an easy matter to eliminate nonsporulating contaminations by heating cultures containing sporulating anaerobes to a temperature sufficient to destroy vegetative forms alone; but selective heating is a total failure for the elimination of hay bacilli, since most of these organisms are less susceptible to heat than