RT Journal A1 Hesseltine H T1 VUlvovaginitis in pregnancy JF Journal of the American Medical Association JO Journal of the American Medical Association YR 1939 FD October 14 VO 113 IS 16 SP 1508 OP 1508 DO 10.1001/jama.1939.02800410058025 UL http://dx.doi.org/10.1001/jama.1939.02800410058025 AB To the Editor:—  "The Significance of Vulvovaginitis in Pregnancy" by Edward G. Waters and Eakle W. Cartwright (The Journal, July 1, p. 30) does not cover the entire subject; certain points deserve elaboration:Many, if not most, researches of recent date have used Sabouraud's or modified Sabouraud's medium directly for isolation of the yeastlike fungi instead of the method employed by these authors. Their technics might explain this low incidence. Surely those interested in this work would appreciate the authority for the technic advocated in this article. Yeast-like fungi were isolated from 32 per cent of gravid women without vulval symptoms by Plass and his co-workers (Am. J. Obst. & Gynec. 21:320 [March] 1931), from 23 per cent of all pregnant women by Weinstein and Wickerham (Yale J. Biol. & Med. 10:553 [July] 1938) and from 14 to 41 per cent in different economic and hygienic groups by Woodruff