RT Journal A1 Hampton T T1 SPermless malaria mosquitoes JF JAMA JO JAMA YR 2011 FD October 5 VO 306 IS 13 SP 1431 OP 1431 DO 10.1001/jama.2011.1390 UL http://dx.doi.org/10.1001/jama.2011.1390 AB The researchers developed spermless male Anopheles gambiae sensu stricto mosquitoes (the species that is the major malaria vector) by silencing the zpg germ cell differentiation gene. Females mated with such spermless males and underwent typical behavioral changes such as egg laying after consumption of a blood meal and an unwillingness to mate again, but the eggs were unfertilized and did not produce offspring. (After mating, females of this species tend to not mate again.)