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Editorial |

Combined T4 and T3 Therapy—Back to the Drawing Board

David S. Cooper, MD
JAMA. 2003;290(22):3002-3004. doi:10.1001/jama.290.22.3002
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Hormone replacement therapy" was originally reported by Murray, who in 1891 described the successful treatment of myxedema with injections of sheep thyroid extract.1 By 1898, therapy for this chronic, severely debilitating condition was hailed as "unparalleled by anything in the whole range of curative measures" by Osler.2 Seven decades later, desiccated thyroid was characterized by the standard thyroid textbook of the day as ". . . as perfect a form of therapy as any known to medicine. . . ."3 With the subsequent identification of thyroxine (T4) and triiodothyronine (T3) as the 2 major thyroid hormones, it was assumed that replacement therapies containing both compounds were more physiological than treatment with either alone. Thus, despite the availability of synthetic T4 in the early 1960s, the prescription of desiccated animal thyroid or synthetic combinations of T4 and T3 continued well into the 1970s.

However, following the report in 1970 that T4 was deiodinated in peripheral tissues to form the more biologically active T3,4 it was realized that T4 monotherapy would yield normal circulating levels of both T4and T3.5 This fact, coupled with concerns about iatrogenic hyperthyroidism from a surge in serum T3 levels following the ingestion of T3-containing preparations, as well as variability in the hormonal content of desiccated thyroid, led to a progressive decrease in their use throughout the 1970s and 1980s.6 Review articles and guidelines on thyroid hormone therapy written in the 1990s all recommend synthetic T4 alone as the treatment of choice for patients with hypothyroidism.7 10 This advice was supported by a small study showing that patients treated with synthetic T4 alone have a quality of life that is similar to that of individuals without a history of thyroid disease.11

Nevertheless, virtually all primary care physicians have in their practices hypothyroid patients with persistent symptoms, despite adequate doses of T4 that are "proven" to be sufficient by normal serum thyroid hormone and thyroid-stimulating hormone (TSH [thyrotropin]) concentrations. This all too frequent scenario was quantified in a recent study showing that hypothyroid patients treated with T4 alone do not score as well on validated scales of psychological well-being and have more hypothyroid symptoms compared with euthyroid controls, despite having normal thyroid function results.12 Whether this finding represents the inability of T4 monotherapy to reproduce the exact hormonal milieu of a euthyroid individual or whether it is the result of having been "labeled" as having an illness (ie, hypothyroidism) remains uncertain.13 14

The use of T4 monotherapy as the treatment of choice for hypothyroidism was challenged in 1999 by a study comparing T4 alone with a combination of T4 and T3.15 In a randomized, blinded crossover design, hypothyroid patients taking the combination of T4 and T3 for 5 weeks had improved cognitive performance, mood, and physical well-being compared with a similar period when they received T4 alone. A post hoc analysis of these data suggested that most of the improvement occurred in athyreotic thyroid cancer patients rather than in patients with autoimmune thyroid disease, who presumably had some residual thyroid function.16 The authors proposed that a combination of T4 and T3 might be a more physiological form of therapy than T4 alone, particularly in individuals without a source of endogenous thyroid hormone. This conclusion has some experimental support; in thyroidectomized rats, T4 and T3 concentrations in a variety of tissues cannot be normalized by any dose of T4, but they can be restored to normal with a combination of both hormones.17 The only exception is the brain, where, because of a very active central nervous system deiodinase, T4 treatment alone yields normal brain levels of T3 over a wide range of administered T4 doses.18

The idea that T4 monotherapy might be a less effective treatment than a combination of T4 and T3 was greeted with skepticism by some.19 On the other hand, symptomatic patients felt a sense of vindication, particularly those who hoped that their persistent hypothyroid symptoms might finally be alleviated with T4 and T3 combination treatments considered by many physicians to be obsolete, ie, "natural" desiccated thyroid or combinations of synthetic T4 plus T3.20

In this issue of THE JOURNAL, Clyde and colleagues21 have attempted to reproduce the results that were reported in 1999, using a randomized parallel-group study design. These investigators intentionally avoided the crossover design used in the original study to eliminate the possibility that performance on psychometric testing could be influenced by a "practice effect." Forty-six hypothyroid patients were enrolled, most of whom had thyroid failure from autoimmune (Hashimoto) thyroiditis. They were randomized to continue to receive either their current dose of T4 or to receive 50 µg less than their current T4 dose, the deficit replaced by T3 at a dose of 7.5 µg twice daily (equivalent metabolically to 50 µg of T4). The authors were careful to adjust the T4 doses in each group to maintain normal TSH levels as best they could throughout the 4-month observation period. At the end of the study, there were no differences between the 2 groups in body weight, blood pressure, or lipid levels. There were also no significant differences in any of the standardized tests that were administered, including a validated hypothyroid symptom scale and a battery of neurocognitive tests.

Interpretation of these negative results is made difficult by the significant improvement in the hypothyroid symptom score in the "control" group, who simply continued to take their usual dose of T4. Why this would be so is uncertain, but it emphasizes the power of the placebo effect. Another problem with the current study, as well as with the 1999 study, is that the doses of T4 and T3 used do not replicate the ratio of T4 to T3 secreted by the normal thyroid gland (14:1 molar ratio).22 Nevertheless, free T4, T3, and TSH levels were normal throughout the study in the majority of patients. This is important, since serum TSH levels were subnormal in some patients in the original study, suggesting that they were made iatrogenically hyperthyroid on the T4 plus T3 combination, a circumstance that has been associated experimentally with an improved sense of well-being.23

Within the last 2 months, 2 additional studies have been published that also failed to confirm the benefits of combined T4 and T3 therapy.24 25 In one report, 110 hypothyroid patients were randomized in a crossover design to receive their usual T4 dosage or 50 µg less than their usual dosage of T4 plus 10 µg of T3.24 Each treatment period lasted for 10 weeks, with a 4-week washout between treatment periods. No changes in cognitive function, quality of life, or hypothyroid symptoms were observed. In the other report, 40 hypothyroid individuals with depressive symptoms who were receiving stable doses of T4 were randomized to continue to receive their chronic dose of T4 or one half of their T4 dose plus 12.5 µg of T3 twice a day.25 No improvements in hypothyroid symptoms or mood were noted after 15 weeks of observation.

Given these 3 negative studies, it would appear, at least superficially, that the putative beneficial role of combined T4 plus T3 therapy cannot be substantiated. However, none of these studies enrolled a large number of patients with surgical or postablative hypothyroidism. Therefore, it remains a possibility that patients with no endogenous thyroid function may benefit from combined therapy, even if patients with residual thyroid secretory capacity do not, as suggested by Bunevicius and Prange.16 Also, none of the 4 published studies used a T4 plus T3 combination that precisely matched the normal hormonal secretion of the thyroid gland. Finally, a long-acting or "slow-release" form of T3, which does not currently exist commercially, would be required to mimic normal physiological endogenous T3 production. Therefore, until studies are performed in thyroidectomized individuals using a combination of drugs formulated at the correct ratio containing a slow-release form of T3, the hypothesis that combination therapy might be superior to T4 monotherapy cannot be completely rejected.

From a practical point of view, autoimmune thyroiditis is the cause of hypothyroidism in the majority of patients living in iodine-sufficient areas of the world.26 Given the negative results from the study by Clyde et al21 and the other 2 recently published controlled trials,24 25 combination treatment cannot be recommended. Treatment of patients with thyroid failure who have persistent hypothyroid or depressive symptoms while taking an adequate dose of T4 (defined by serum TSH levels between 0.5 and 2.5 mIU/L27 ) remains a major challenge. However, combined T4 plus T3 is not the answer.

After more than a century of successful treatment of hypothyroidism, it is fascinating that such seemingly simple hormonal replacement therapy would be the subject of so much continuing controversy. Before another century elapses, let us hope that the optimum treatment for all hypothyroid patients will be revealed by additional well-designed clinical trials using novel thyroid hormone preparations that precisely mirror normal thyroid physiology. Then, and only then, will thyroid replacement be ". . . as perfect a form of therapy as any known to medicine."3

REFERENCES

Murray GR. Note on the treatment of myxoedema by hypodermic injections of an extract of the thyroid gland of a sheep.  BMJ.1891;2:796-797.
Osler W. The Principles and Practice of Medicine. 3rd ed. New York, NY: Appleton; 1898.
Means JH, DeGroot LJ, Stanbury JB. The Thyroid and Its Diseases. 3rd ed. New York, NY: McGraw-Hill; 1963.
Braverman LE, Ingbar SH, Sterling K. Conversion of thyroxine (T4) to triiodothyronine (T3) in athyreotic human subjects.  J Clin Invest.1970;49:855-864.
PubMed
Surks MI, Schadlow AR, Oppenheimer JH. A new radioimmunoassay for plasma L-triiodothyronine: measurements in thyroid disease and in patients maintained on hormonal replacement.  J Clin Invest.1972;51:3104-3113.
PubMed
Kaufman SC, Gross TP, Kennedy DL. Thyroid hormone use: trends in the United States from 1960 through 1988.  Thyroid.1991;1:285-291.
PubMed
Mandel SJ, Brent GA, Larsen PR. Levothyroxine therapy in patients with thyroid disease.  Ann Intern Med.1993;119:492-502.
PubMed
Toft AD. Thyroxine therapy.  N Engl J Med.1994;331:174-180.
PubMed
Roti E, Minelli R, Gardini E, Braverman LE. The use and misuse of thyroid hormone.  Endocr Rev.1993;14:401-423.
PubMed
Singer PA, Cooper DS, Levy EG.  et al. for the Standards of Care Committee, American Thyroid Association.  Treatment guidelines for patients with hyperthyroidism and hypothyroidism.  JAMA.1995;273:808-812.
PubMed
Petersen K, Bengtsson C, Lapidus L, Lindstedt G, Nystrom E. Morbidity, mortality, and quality of life for patients treated with levothyroxine.  Arch Intern Med.1990;150:2077-2081.
PubMed
Saravanan P, Chau WF, Roberts N, Vedhara K, Greenwood R, Dayan CM. Psychological well-being in patients on "adequate" doses of l-thyroxine: results of a large, controlled community-based questionnaire study.  Clin Endocrinol (Oxf).2002;57:577-585.
PubMed
Walsh JP. Dissatisfaction with thyroxine therapy—could the patients be right?  Curr Opin Pharmacol.2002;2:717-722.
PubMed
Ladenson PW. Psychological wellbeing in patients.  Clin Endocrinol (Oxf).2002;57:575-576.
PubMed
Bunevicius R, Kazanavicius G, Zalinkevicius R, Prange Jr AJ. Effects of thyroxine as compared with thyroxine plus triiodothyronine in patients with hypothyroidism.  N Engl J Med.1999;340:424-429.
PubMed
Bunevicius R, Prange AJ. Mental improvement after replacement therapy with thyroxine plus triiodothyronine: relationship to cause of hypothyroidism.  Int J Neuropsychopharmacol.2000;3:167-174.
PubMed
Escobar-Morreale HF, Obregon MJ, Escobar del Rey F, Morreale de Escobar G. Replacement therapy for hypothyroidism with thyroxine alone does not ensure euthyroidism in all tissues, as studied in thyroidectomized rats.  J Clin Invest.1995;96:2828-2838.
PubMed
Escobar-Morreale HF, Obregon MJ, Hernandez A, Escobar del Rey F, Morreale de Escobar G. Regulation of iodothyronine deiodinase activity as studied in thyroidectomized rats infused with thyroxine or triiodothyronine.  Endocrinology.1997;138:2559-2568.
PubMed
Toft AD. Thyroid hormone replacement—one hormone or two?  N Engl J Med.1999;340:469-470.
PubMed
Shomon MJ. T3 helps with hypothyroidism/New England Journal. Available at: http://thyroid.about.com/library/weekly/aa021199.htm. Accessed March 4, 2002.
Clyde PW, Harari AE, Getka EJ, Shakir KMM. Combined levothyroxine plus liothyronine compared with levothyroxine alone in primary hypothyroidism: a randomized controlled trial.  JAMA.2003;290:2952-2958.
Pilo A, Iervasi G, Vitek F, Ferdeghini M, Cazzuola F, Bianchi R. Thyroidal and peripheral production of 3,5,3'-triiodothyronine in humans by multicompartmental analysis.  Am J Physiol.1990;258(4 pt 1):E715-E726.
PubMed
Carr D, McLeod DT, Parry G, Thornes HM. Fine adjustment of thyroxine replacement dosage: comparison of the thyrotrophin releasing hormone test using a sensitive thyrotrophin assay with measurement of free thyroid hormones and clinical assessment.  Clin Endocrinol (Oxf).1988;28:325-333.
PubMed
Walsh JP, Shiels L, Lim EM.  et al.  Combined thyroxine/liothyronine treatment does not improve well-being, quality of life or cognitive function compared to thyroxine alone: a randomized controlled trial in patients with primary hypothyroidism.  J Clin Endocrinol Metab.2003;88:4543-4550.
PubMed
Sawka AM, Gerstein HC, Marriott MJ, MacQueen GM, Joffe RT. Does a combination of T4 and T3 improve depressive symptoms better than T4 alone in patients with hypothyroidism? results of a double-blinded randomized controlled trial.  J Clin Endocrinol Metab.2003;88:4551-4555.
PubMed
Ladenson PW. Problems in the management of hypothyroidism. In: Braverman LE, ed. Diseases of the Thyroid. 2nd ed. Totowa, NJ: Humana Press; 2002:161-176.
American Association of Clinical Endocrinologists.  Medical guidelines for clinical practice for the evaluation and treatment of hyperthyroidism and hypothyroidism.  Endocr Pract2002;8:457-467.

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Murray GR. Note on the treatment of myxoedema by hypodermic injections of an extract of the thyroid gland of a sheep.  BMJ.1891;2:796-797.
Osler W. The Principles and Practice of Medicine. 3rd ed. New York, NY: Appleton; 1898.
Means JH, DeGroot LJ, Stanbury JB. The Thyroid and Its Diseases. 3rd ed. New York, NY: McGraw-Hill; 1963.
Braverman LE, Ingbar SH, Sterling K. Conversion of thyroxine (T4) to triiodothyronine (T3) in athyreotic human subjects.  J Clin Invest.1970;49:855-864.
PubMed
Surks MI, Schadlow AR, Oppenheimer JH. A new radioimmunoassay for plasma L-triiodothyronine: measurements in thyroid disease and in patients maintained on hormonal replacement.  J Clin Invest.1972;51:3104-3113.
PubMed
Kaufman SC, Gross TP, Kennedy DL. Thyroid hormone use: trends in the United States from 1960 through 1988.  Thyroid.1991;1:285-291.
PubMed
Mandel SJ, Brent GA, Larsen PR. Levothyroxine therapy in patients with thyroid disease.  Ann Intern Med.1993;119:492-502.
PubMed
Toft AD. Thyroxine therapy.  N Engl J Med.1994;331:174-180.
PubMed
Roti E, Minelli R, Gardini E, Braverman LE. The use and misuse of thyroid hormone.  Endocr Rev.1993;14:401-423.
PubMed
Singer PA, Cooper DS, Levy EG.  et al. for the Standards of Care Committee, American Thyroid Association.  Treatment guidelines for patients with hyperthyroidism and hypothyroidism.  JAMA.1995;273:808-812.
PubMed
Petersen K, Bengtsson C, Lapidus L, Lindstedt G, Nystrom E. Morbidity, mortality, and quality of life for patients treated with levothyroxine.  Arch Intern Med.1990;150:2077-2081.
PubMed
Saravanan P, Chau WF, Roberts N, Vedhara K, Greenwood R, Dayan CM. Psychological well-being in patients on "adequate" doses of l-thyroxine: results of a large, controlled community-based questionnaire study.  Clin Endocrinol (Oxf).2002;57:577-585.
PubMed
Walsh JP. Dissatisfaction with thyroxine therapy—could the patients be right?  Curr Opin Pharmacol.2002;2:717-722.
PubMed
Ladenson PW. Psychological wellbeing in patients.  Clin Endocrinol (Oxf).2002;57:575-576.
PubMed
Bunevicius R, Kazanavicius G, Zalinkevicius R, Prange Jr AJ. Effects of thyroxine as compared with thyroxine plus triiodothyronine in patients with hypothyroidism.  N Engl J Med.1999;340:424-429.
PubMed
Bunevicius R, Prange AJ. Mental improvement after replacement therapy with thyroxine plus triiodothyronine: relationship to cause of hypothyroidism.  Int J Neuropsychopharmacol.2000;3:167-174.
PubMed
Escobar-Morreale HF, Obregon MJ, Escobar del Rey F, Morreale de Escobar G. Replacement therapy for hypothyroidism with thyroxine alone does not ensure euthyroidism in all tissues, as studied in thyroidectomized rats.  J Clin Invest.1995;96:2828-2838.
PubMed
Escobar-Morreale HF, Obregon MJ, Hernandez A, Escobar del Rey F, Morreale de Escobar G. Regulation of iodothyronine deiodinase activity as studied in thyroidectomized rats infused with thyroxine or triiodothyronine.  Endocrinology.1997;138:2559-2568.
PubMed
Toft AD. Thyroid hormone replacement—one hormone or two?  N Engl J Med.1999;340:469-470.
PubMed
Shomon MJ. T3 helps with hypothyroidism/New England Journal. Available at: http://thyroid.about.com/library/weekly/aa021199.htm. Accessed March 4, 2002.
Clyde PW, Harari AE, Getka EJ, Shakir KMM. Combined levothyroxine plus liothyronine compared with levothyroxine alone in primary hypothyroidism: a randomized controlled trial.  JAMA.2003;290:2952-2958.
Pilo A, Iervasi G, Vitek F, Ferdeghini M, Cazzuola F, Bianchi R. Thyroidal and peripheral production of 3,5,3'-triiodothyronine in humans by multicompartmental analysis.  Am J Physiol.1990;258(4 pt 1):E715-E726.
PubMed
Carr D, McLeod DT, Parry G, Thornes HM. Fine adjustment of thyroxine replacement dosage: comparison of the thyrotrophin releasing hormone test using a sensitive thyrotrophin assay with measurement of free thyroid hormones and clinical assessment.  Clin Endocrinol (Oxf).1988;28:325-333.
PubMed
Walsh JP, Shiels L, Lim EM.  et al.  Combined thyroxine/liothyronine treatment does not improve well-being, quality of life or cognitive function compared to thyroxine alone: a randomized controlled trial in patients with primary hypothyroidism.  J Clin Endocrinol Metab.2003;88:4543-4550.
PubMed
Sawka AM, Gerstein HC, Marriott MJ, MacQueen GM, Joffe RT. Does a combination of T4 and T3 improve depressive symptoms better than T4 alone in patients with hypothyroidism? results of a double-blinded randomized controlled trial.  J Clin Endocrinol Metab.2003;88:4551-4555.
PubMed
Ladenson PW. Problems in the management of hypothyroidism. In: Braverman LE, ed. Diseases of the Thyroid. 2nd ed. Totowa, NJ: Humana Press; 2002:161-176.
American Association of Clinical Endocrinologists.  Medical guidelines for clinical practice for the evaluation and treatment of hyperthyroidism and hypothyroidism.  Endocr Pract2002;8:457-467.
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