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To the Editor: Urinary tract infections (UTIs) account for more than 11 million physician visits annually in the United States and have become increasingly resistant to first-line antibiotic therapy.1 Recent evidence suggests that consumption of cranberry juice beverages is effective at preventing UTIs,2 - 3 although further studies are needed to validate potential treatment effects. While early research focused on a mechanism of urinary acidification, the largest clinical trial to date found no evidence to support this.2 Recent studies suggest that cranberry proanthocyanidins (condensed tannins) may inhibit P-fimbriated Escherichia coli from adhering to uroepithelial cells,4 the initial step in development of UTI. The effectiveness of cranberry proanthocyanidins and cranberry beverages against antibiotic-resistant E coli, however, has not been previously tested. We assessed whether consumption of cranberry juice cocktail prevents adhesion of antibiotic-resistant uropathogenic P-fimbriated E coli to the uroepithelium.
Thirty-nine uropathogenic P-fimbriated E coli isolates were obtained from women aged 18 to 39 years with clinically diagnosed, culture-confirmed UTIs. Isolates were incubated for 20 minutes in urine collected over a 12-hour period from healthy women before and after consumption of 240 mL of commercial cranberry juice cocktail, and in cranberry proanthocyanidin extract (pH 6.5) (2-fold dilution series). Isolates tested were a subset of those previously screened for resistance; 24 (62%) of those selected were resistant to trimethoprim-sulfamethoxazole.5 These bacteria were then harvested and screened for ability to adhere to isolated uroepithelial cells, agglutinate human red blood cells (A1, Rh+), and resin beads coated with isolated P-receptor oligosaccharides.
Urine after cranberry juice cocktail consumption (average pH 6.2) prevented adhesion of 31 (80%) of the 39 isolates and 19 (79%) of the 24 antibiotic-resistant isolates in all bioassays, while preconsumption urine (average pH 6.2) failed to prevent adhesion in any of the samples. Antiadhesion activity was evident in the urine within 2 hours and persisted for up to 10 hours following cranberry juice cocktail ingestion. The extracted proanthocyanidins inhibited adhesion of all isolates at concentrations ranging from 6 to 375 µg/mL, demonstrating potent in vitro antiadhesion activity against these antibiotic-resistant strains.
These data suggest that consumption of cranberry juice cocktail may offer protection against both sensitive and resistant strains of P-fimbriated E coli by a mechanism that is not likely to increase selective pressures associated with antibiotic resistance. In light of the evidence that antibiotic usage is a contributing factor in development of trimethoprim-sulfamethoxazole–resistant uropathogenic E coli,5 further trials are warranted to explore the use of cranberry juice as an alternative strategy to prevent UTIs and potentially reduce the rate of antibiotic resistance.
Funding/Support: Isolate collection was supported by National Institutes of Health grant R01 DK35368, and bacterial antiadhesion work was supported by Ocean Spray Cranberries, Inc. Ocean Spray provided monetary support only and did not participate in the design, analysis, or interpretation of the data.
Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature
Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal
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