Genital Human Papillomavirus Infection in Female University Students as Determined by a PCR-Based Method FREE

Heidi M. Bauer; Yi Ting, MS; Catherine E. Greer; Janet C. Chambers, MD; Cathy J. Tashiro, MPH; Joseph Chimera, PhD; Arthur Reingold, MD; M. Michele Manos, PhD

[+] Author Affiliations

Reprint requests and correspondence to Cetus Corp, 1400 53rd St, Emeryville, CA94608 (Dr Manos).

JAMA. 1991;265(4):472-477. doi:10.1001/jama.1991.03460040048027

Text Size: A A A

Published online

Article

References

ABSTRACT

ABSTRACT | REFERENCES

The presence of genital human papillomavirus (HPV) was determined at cervical and vulvar sites using two methods, the Food and Drug Administration—approved Vira Pap test and polymerase chain reaction (PCR) DNA amplification technology, in 467 women presenting to a university health service for a routine annual gynecologic examination. The PCR system afforded the sensitive detection of a broad spectrum of genital HPV types. Using PCR, we found that 46% of the study population was infected with HPV; the ViraPap test showed a prevalence of 11% infected. PCR analyses demonstrated that 69% of the HPV-positive women were infected at both genital sites. Subsequent HPV-type determination showed that 33% of the study population had HPV types 6, 11, 16, 18,31,33,35,39,45,51,52, or other previously isolated types, and 13% had yet unidentified types. Almost all (92%) of the women diagnosed by Papanicolaou smear with condylomatous atypia or dysplasia (n = 12) were HPV positive. The PCR method proved to be an informative and rapid way to detect HPV in large numbers of clinical samples. Our results demonstrate that genital HPV infection is common among sexually active young women.

(JAMA. 1991;265:472-477)

REFERENCES

ABSTRACT | REFERENCES

Koutsky LA, Galloway DA, Holmes KK. Epidemiology of genital human papillomavirus infection . Epidemiol Rev. 1988;;10:122-163.

Roman A, Fife KH. Human papillomaviruses: are we ready to type? Clin Microbiol Rev. 1989;;2:166-190.

Kiviat NB, Koutsky LA, Paavonen JA, et al. Prevalence of genital papillomavirus infection among women attending a college student health clinic or a sexually transmitted diseases clinic . J Infect Dis. 1989;;159:293-302.

Lorincz AT, Schiffman MH, Jaffurs WJ, Marlow J, Quinn AP, Temple GF. Temporal associations of human papillomavirus infection with cervical cytological abnormalities . Am J Obstet Gynecol. 1990;;162:645-651.

Reeves WC, Arosemena JR, Garcia M, et al. Genital human papillomavirus infection in Panama City prostitutes . J Infect Dis. 1989;;160:599-603.

Reeves WC, Brinton LA, Garcia M, et al. Human papillomavirus infection and cervical cancer in Latin America . N Engl J Med. 1989;;320:1437-1441.

Rosenfeld WD, Vermund SH, Wentz SJ, Burk RD. High prevalence rate of human papillomavirus infection and association with abnormal Papanicolaou smears in sexually active adolescents . AJDC . 1989;;143:1443-1447.

Villa LL, Franco ELF. Epidemiologic correlates of cervical neoplasia and risk of human papillomavirus infection in asymptomatic women in Brazil . J Natl Cancer Inst. 1989;;81:332-340.

Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction . Methods Enzymol. 1987;;155:335-350.

Saiki RK, Scharf S, Faloona F, et al. Enzymatic amplification of β-globin genomic sequence and restriction site analysis for diagnosis of sickle cell anemia . Science . 1985;;230:1350-1354.

Ferre F, Garduno F. Detection of human papillomavirus types 6/11, 16, and 18 using the polymerase chain reaction . Cancer Cells 7, Molecular Diagnostics of Human Cancer . New York, NY: Cold Spring Harbor Laboratory Press; 1989;:215-218.

Manos MM, Ting Y, Wright DK, Lewis AJ, Broker TR, Wolinsky SM. Use of polymerase chain reaction amplification for the detection of genital human papillomaviruses . Cancer Cells 7, Molecular Diagnostics of Human Cancer . New York, NY: Cold Spring Harbor Laboratory Press; 1989;:209-214.

Pao CC, Lin C-Y, Maa J-S, Lai C-H, Wu S-Y, Soong Y-K. Detection of human papillomaviruses in cervicovaginal cells using polymerase chain reaction . J Infect Dis. 1990;;161:113-115.

Shibata D, Arnheim N, Martin WJ. Detection of human papillomavirus in paraffin-embedded tissue using the polymerase chain reaction . J Exp Med. 1988;;167:225-230.

Kiviat NB, Koutsky LA, Critchlow CW, et al. Comparison of Southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33, and 35 . Am J Clin Pathol. 1990;;94:561-565.

Ting Y, Manos MM. Detection and typing of genital human papillomaviruses . In: Innis M, Gelfand D, Sninsky J, White T, eds. PCR Protocols: A Guide to Methods and Applications . San Diego, Calif: Academic Press Inc; 1990;:356-367.

Resnick RM, Cornelissen MTE, Wright DK, et al. Detection and typing of HPV in archival cervical cancer specimens using DNA amplification with consensus primers . J Natl Cancer Inst. 1990;; 82:1477-1484.

Schiffman MH, Bauer HM, Lorincz AT, et al. A comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA . J Clin Microbiol. In press.

Gregoire L, Arella M, Campione-Piccardo J, Lancaster WD. Amplification of human papillomavirus DNA sequences by using conserved primers . J Clin Microbiol. 1989;;27:2660-2665.

Snijders PJF, van de Brule AJC, Schrijnemakers HFJ, Snow G, Meijer CJLM, Walboomers JMM. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes . J Gen Virol. 1990;;71:173-181.

National Cancer Institute Workshop. The 1988 Bethesda System for reporting cervical/vaginal cytologic diagnoses . JAMA . 1989;;262:931-934.

Kwok S, Higuchi R. Avoiding false positives with PCR . Nature . 1989;;339:237-238.

Maniatis T, Fritsch EF, Sambrook J. Molecular Cloning: A Laboratory Manual . New York, NY: Cold Spring Harbor Laboratory Press; 1982;:122-123.

Tidy JA, Vousden KH, Farrell PJ. Relation between infection with a subtype of HPV16 and cervical neoplasia . Lancet . 1989;;1:1225-1227.

Ward P, Parry GN, Yule R, Coleman DV, Malcolm ADB. Human papillomavirus subtype 16a . Lancet . 1989;;1:170.

Young LS, Bevan IS, Johnson MA, et al. The polymerase chain reaction: a new epidemiological tool for investigating cervical human papillomavirus infection . BMJ . 1989;;298:14-18.

Tidy J, Farrell PJ. Retraction: human paillomavirus subtype 16b . Lancet . 1989;;2:1535.

Manos M, Lee K, Greer C, et al. Looking for human papillomavirus type 16 by PCR . Lancet . 1990;;335:734.

Kjaer SK, Engholm G, Teisen C, et al. Risk factors for cervical human papillomavirus and herpes simplex virus infections in Greenland and Denmark: a population-based study . Am J Epidemiol. 1990;;131:669-682.

Manos M. Prevalence of genital HPV infection in female university students determined by a PCR-based method . Presented at the Papillomavirus Workshop; May 16, 1990 ; Heidelberg, Germany.

de Villiers E-M. Heterogeneity of the human papillomavirus group . J Virol. 1989;;63:4898-4903.

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal