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April 28, 1975, Vol 232, No. 4 >
ARTICLE | April 28, 1975

Acute Viral Hepatitis in Adults: Title and subTitle BreakComparison of the Radioimmunoassay and Counterimmunoelectrophoresis Methods of Detecting HBsAg FREE

Richard P. Wenzel, MD; C. David Teates, MD; Querido Galapon, RT; Robert Barczak, RT; Chung-mei Ling, PhD; Lacy R. Overby, PhD
[+] Author Affiliations

Reprint requests to Department of Internal Medicine, University of Virginia School of Medicine, PO Box 370, Charlottesville, VA 22901 (Dr. Wenzel).


JAMA. 1975;232(4):366-368. doi:10.1001/jama.1975.03250040020019
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References

We compared the radioimmunoassay (RIA) and counterimmunoelectrophoretic (CIE) methods in detecting hepatitis B antigen (HB Ag) in 407 acute and 336 convalescent sera of adults with viral hepatitis. The CIE method demonstrated that 41% of acute and 28% of 14- to 17-day serum specimens were HBsAg-positive. The RIA method demonstrated seropositivity in 60% of acute and 56% of convalescent specimens (P <.001). Eightyfour percent of coded specimens initially positive for HB Ag by RIA were found to have subtype antigenic determinants d or y; 92% of the HB Ag-negative controls were negative for subtype antigens, confirming the specificity of the RIA test. RIA subtyping data corroborated earlier work with immunodiffusion techniques.

(JAMA 232:366-368, 1975)

REFERENCES

1 +
Blumberg BS:  Polymorphisms of the serum proteins and development of iso-precipitans in transfused patients . Bull NY Acad Med 40:377-386, 1964;.
2 +
Le Bouvier GL, McCollum RW:  Australia (hepatitis-associated) antigen: Physiochemical and immunologic characteristics , in Advances in Virus Research . New York, Academic Press Inc, 1970;, vol 16, pp 357-396.
3 +
Krugman S, Giles JP, Hammond J:  Infectious hepatitis: Evidence for two distinctive clinical, epidemiological, and immunological types of infection . JAMA 200:365-373, 1967;.
4 +
Krugman S, Giles JP:  Viral hepatitis: New light on an old disease . JAMA 212:1019-1029, 1970;.
5 +
Alter HJ, Holland PV, Purcell RH, et al:  Post-transfusion hepatitis after exclusion of commercial and hepatitis B antigen positive donors . Ann Intern Med 77:691-699, 1972;.
6 +
Prince AM, Hargrove RL, Szmuness W, et al:  Immunological distinction between infectious and serum hepatitis . N Engl J Med 282:987-991, 1970;.
7 +
Cossart YE, Vahrman J:  Studies of Australia-SH antigen in sporadic viral hepatitis in London . Br Med J 1:403-405, 1970;.
8 +
Wenzel RP, Le Bouvier GL, Beam WE Jr:  Drug abuse and viral hepatitis in Marines . JAMA 220:707-709, 1972;.
9 +
Ling CM, Overby LR:  Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125I-antibody . J Immunol 109:834-841, 1972;.
10 +
Koretz RL, Klahs DR, Ritman S, et al:  Post-transfusion hepatitis in recipients of blood screened by newer assays . Lancet 2:694-696, 1973;.
11 +
Sgouris JT:  Limitations of the radioimmunoassay for hepatitis B antigen . N Engl J Med 288:160-161, 1973;.
12 +
Hollinger FB, Aach RD, Gitnick GL, et al:  Limitations of solid-phase radioimmunoassay for HB Ag in reducing frequency of post-transfusion hepatitis . N Engl J Med 289:385-391, 1973;.
13 +
Prince AM, Brotman B, Jass D, et al:  Specificity of the direct solid-phase radioimmunoassay for detection of hepatitis-B antigen . Lancet 1:1346-1350, 1973;.
14 +
Le Bouvier GL:  The heterogeneity of Australia antigen . J Infect Dis 102:671-675, 1971;.
15 +
Ling CM, Irace H, Decker R, et al:  Hepatitis B virus antigen: Validation and immunologic characterization of low-titer serums with 125I-antibody . Science 180:203-205, 1973;.
16 +
Blumberg BS, Riddell NM:  Inherited antigenic differences in human serum beta lipoproteins: A second antiserum . J Clin Invest 42:867-875, 1963;.
17 +
Wenzel RP, McCormick DP, Beam WE Jr:  Clinical applications of Australia/hepatitis-associated antigen . South Med J 66:186-189, 1973;.

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Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

1 +
Blumberg BS:  Polymorphisms of the serum proteins and development of iso-precipitans in transfused patients . Bull NY Acad Med 40:377-386, 1964;.
2 +
Le Bouvier GL, McCollum RW:  Australia (hepatitis-associated) antigen: Physiochemical and immunologic characteristics , in Advances in Virus Research . New York, Academic Press Inc, 1970;, vol 16, pp 357-396.
3 +
Krugman S, Giles JP, Hammond J:  Infectious hepatitis: Evidence for two distinctive clinical, epidemiological, and immunological types of infection . JAMA 200:365-373, 1967;.
4 +
Krugman S, Giles JP:  Viral hepatitis: New light on an old disease . JAMA 212:1019-1029, 1970;.
5 +
Alter HJ, Holland PV, Purcell RH, et al:  Post-transfusion hepatitis after exclusion of commercial and hepatitis B antigen positive donors . Ann Intern Med 77:691-699, 1972;.
6 +
Prince AM, Hargrove RL, Szmuness W, et al:  Immunological distinction between infectious and serum hepatitis . N Engl J Med 282:987-991, 1970;.
7 +
Cossart YE, Vahrman J:  Studies of Australia-SH antigen in sporadic viral hepatitis in London . Br Med J 1:403-405, 1970;.
8 +
Wenzel RP, Le Bouvier GL, Beam WE Jr:  Drug abuse and viral hepatitis in Marines . JAMA 220:707-709, 1972;.
9 +
Ling CM, Overby LR:  Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125I-antibody . J Immunol 109:834-841, 1972;.
10 +
Koretz RL, Klahs DR, Ritman S, et al:  Post-transfusion hepatitis in recipients of blood screened by newer assays . Lancet 2:694-696, 1973;.
11 +
Sgouris JT:  Limitations of the radioimmunoassay for hepatitis B antigen . N Engl J Med 288:160-161, 1973;.
12 +
Hollinger FB, Aach RD, Gitnick GL, et al:  Limitations of solid-phase radioimmunoassay for HB Ag in reducing frequency of post-transfusion hepatitis . N Engl J Med 289:385-391, 1973;.
13 +
Prince AM, Brotman B, Jass D, et al:  Specificity of the direct solid-phase radioimmunoassay for detection of hepatitis-B antigen . Lancet 1:1346-1350, 1973;.
14 +
Le Bouvier GL:  The heterogeneity of Australia antigen . J Infect Dis 102:671-675, 1971;.
15 +
Ling CM, Irace H, Decker R, et al:  Hepatitis B virus antigen: Validation and immunologic characterization of low-titer serums with 125I-antibody . Science 180:203-205, 1973;.
16 +
Blumberg BS, Riddell NM:  Inherited antigenic differences in human serum beta lipoproteins: A second antiserum . J Clin Invest 42:867-875, 1963;.
17 +
Wenzel RP, McCormick DP, Beam WE Jr:  Clinical applications of Australia/hepatitis-associated antigen . South Med J 66:186-189, 1973;.

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