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Purified Influenza Virus Vaccine: Title and subTitle BreakA Study of Viral Reactivity and Antigenicity FREE

F. Bruce Peck, Jr., MD
[+] Author Affiliations

Read before the session on large scale antigen purification. Federation of American Societies for Experimental Biology, Atlantic City, NJ, April 17, 1968.

Reprint requests to Lilly Laboratory for Clinical Research, Marion County General Hospital, Indianapolis 46207.


JAMA. 1968;206(10):2277-2282. doi:10.1001/jama.1968.03150100027005
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The development of procedures for the use of the K-II continuous flow zonal ultracentrifuge has resulted, for the first time, in the availability of large quantities of highly purified influenza virus vaccine essentially free of nonviral egg-derived material. With these vaccines, it was determined that the inherent toxicity of purified virus strains and combinations thereof is uniformly low, and that the variable side effects frequently seen with conventionally produced vaccines are not virus related. Purified vaccines are equal in antigenicity to equivalent doses of conventional vaccines, demonstrating that nonviral components of conventional vaccine have no beneficial effects on vaccine antigenicity. Purified vaccine of twice normal potency is no more reactogenic than the same vaccine in standard doses, indicating that future vaccines of even higher potency and low reactivity are well within the realm of possibility.

REFERENCES

Reimer, C.B., et al:  Influenza Virus Purification With the Zonal Ultracentrifuge , Science 152:1379-1381 ( (June 3) ) 1966;.
Reimer, C.B., et al:  Comparison of Techniques for Influenza Virus Purification , J Bact 92:1271-1272 ( (Oct) ) 1966;.
Reimer, C.B., et al:  Purification of Large Quantities on Influenza Virus by Density Gradient Centrifugation , J Virol 1:1207-1216 ( (Dec) ) 1967;.
Baker, R.S.; Van Frank, R.M.; Dodge, R.M.:  Determination of the Purity of Purified Influenza Vaccines , Fed Proc 27:365 ( (March) -April) 1968;.
Public Health Service Regulations: Minimum Requirements for Influenza Virus Vaccine, Types A & B, and Amendments Thereof , US Public Health Service, Division of Biologics Standards, 6th revision, (May 15) , 1947;.
 An Agglutination-Inhibition Test Proposed as a Standard Reference in Influenza Diagnostic Studies, Committee on Standard Serological Procedures in Influenza Studies , J Immunol 65: 347-353 ( (Sept) ) 1950;.

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Reimer, C.B., et al:  Influenza Virus Purification With the Zonal Ultracentrifuge , Science 152:1379-1381 ( (June 3) ) 1966;.
Reimer, C.B., et al:  Comparison of Techniques for Influenza Virus Purification , J Bact 92:1271-1272 ( (Oct) ) 1966;.
Reimer, C.B., et al:  Purification of Large Quantities on Influenza Virus by Density Gradient Centrifugation , J Virol 1:1207-1216 ( (Dec) ) 1967;.
Baker, R.S.; Van Frank, R.M.; Dodge, R.M.:  Determination of the Purity of Purified Influenza Vaccines , Fed Proc 27:365 ( (March) -April) 1968;.
Public Health Service Regulations: Minimum Requirements for Influenza Virus Vaccine, Types A & B, and Amendments Thereof , US Public Health Service, Division of Biologics Standards, 6th revision, (May 15) , 1947;.
 An Agglutination-Inhibition Test Proposed as a Standard Reference in Influenza Diagnostic Studies, Committee on Standard Serological Procedures in Influenza Studies , J Immunol 65: 347-353 ( (Sept) ) 1950;.
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