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ARTICLE |

Culture of Normal Human Leukocytes FREE

George E. Moore, MD, PhD; Robert E. Gerner, MD; H. Addison Franklin
[+] Author Affiliations

Reprint requests to 666 Elm St, Buffalo 14203 (Dr. Moore).


From the Department of Surgery, Roswell Park Memorial Institute, Buffalo.


JAMA. 1967;199(8):519-524. doi:10.1001/jama.1967.03120080053007
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Rapidly growing and apparently permanent cultures were established from hematopoietic cells from two staff members with no known malignant disease. The cell lines were similar to those derived from leukocytes from the peripheral blood of patients with malignancies of either hematopoietic or nonhematopoietic origin and to the lymphoblastoid cell lines derived from Burkitt's lymphoma. Both cell lines contained a herpetic-like virus (leukovirus), produced immunoglobulins, and grew rapidly in various suspension cultures.

REFERENCES

Moore, G.E., et al:  Culture of Human Leukemia Cells , Cancer 19:713-723 ( (May) ) 1966;.
Moore, G.E., et al:  Leukocyte Cultures of Patients With Leukemia and Lymphomas , New York J Med 66:2757-2764 ( (Nov 1) ) 1966;.
Foley, G.E., et al:  Continuous Culture of Human Lymphoblasts From Peripheral Blood of a Child With Acute Leukemia , Cancer 18:522-529 ( (April) ) 1965;.
Epstein, M.A., and Barr, Y.M.:  Characteristics and Mode of Growth of a Tissue Culture Strain (EB1) of Human Lymphoblasts From Burkitt's Lymphoma , J Nat Cancer Inst 34:231-240 ( (Feb) ) 1965;.
Moore, G.E., and Ulrich, K.:  Suspension Cultures of Mammalian Cells , J Surg Res 5:270-282 ( (June) ) 1965;.
Hirschhorn, K.; Bach, F.; and Kolodny, R.L.:  Immune Response and Mitosis of Human Peripheral Blood Lymphocytes In Vitro , Science 142:1185-1187 ( (Nov) ) 1963;.
Bach, F.H., and Hirschhorn, K.:  The In Vitro Immune Response of Peripheral Blood Lymphocytes , Seminars Hemat 2:68-89 ( (Jan) ) 1965;.
Pulvertaft, R.J.:  Cytology of Burkitt's Tumor (African Lymphoma) , Lancet 1:238-240 ( (Feb) ) 1964;.
Toplin, I., and Schidlovsky, G.:  Partial Purification and Electron Microscopy of Virus in the EB-3 Cell Line Derived From a Burkitt Lymphoma , Science 152:1084-1085 ( (May 20) ) 1966;.
Toshima, S., et al:  Electron Microscopic and Cytogenetic Studies of Cells Derived From Burkitt's Lymphoma , Cancer Res , to be published.
Tanigaki, N., et al:  Immunoglobulin Production in Human Leukemia Cell Lines , J Immunol 97:634-646 ( (Nov) ) 1966;.
Fahey, J.L., et al:  Immunoglobulin Synthesis In Vitro by Established Human Cell Lines , Science 152:1259-1261 ( (May 27) ) 1966;.
Iwakata, S.. and Grace, J.T.:  Cultivation In Vitro of Myeloblasts From Human Leukemia , New York J Med 64:2279-2282 ( (Sept) ) 1964;.
Grace, J.T.:  Recent Studies of Human Leukemia , read before the 20th Annual Symposium on Fundamental Cancer Research at the University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, March 7,1966 .

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Moore, G.E., et al:  Culture of Human Leukemia Cells , Cancer 19:713-723 ( (May) ) 1966;.
Moore, G.E., et al:  Leukocyte Cultures of Patients With Leukemia and Lymphomas , New York J Med 66:2757-2764 ( (Nov 1) ) 1966;.
Foley, G.E., et al:  Continuous Culture of Human Lymphoblasts From Peripheral Blood of a Child With Acute Leukemia , Cancer 18:522-529 ( (April) ) 1965;.
Epstein, M.A., and Barr, Y.M.:  Characteristics and Mode of Growth of a Tissue Culture Strain (EB1) of Human Lymphoblasts From Burkitt's Lymphoma , J Nat Cancer Inst 34:231-240 ( (Feb) ) 1965;.
Moore, G.E., and Ulrich, K.:  Suspension Cultures of Mammalian Cells , J Surg Res 5:270-282 ( (June) ) 1965;.
Hirschhorn, K.; Bach, F.; and Kolodny, R.L.:  Immune Response and Mitosis of Human Peripheral Blood Lymphocytes In Vitro , Science 142:1185-1187 ( (Nov) ) 1963;.
Bach, F.H., and Hirschhorn, K.:  The In Vitro Immune Response of Peripheral Blood Lymphocytes , Seminars Hemat 2:68-89 ( (Jan) ) 1965;.
Pulvertaft, R.J.:  Cytology of Burkitt's Tumor (African Lymphoma) , Lancet 1:238-240 ( (Feb) ) 1964;.
Toplin, I., and Schidlovsky, G.:  Partial Purification and Electron Microscopy of Virus in the EB-3 Cell Line Derived From a Burkitt Lymphoma , Science 152:1084-1085 ( (May 20) ) 1966;.
Toshima, S., et al:  Electron Microscopic and Cytogenetic Studies of Cells Derived From Burkitt's Lymphoma , Cancer Res , to be published.
Tanigaki, N., et al:  Immunoglobulin Production in Human Leukemia Cell Lines , J Immunol 97:634-646 ( (Nov) ) 1966;.
Fahey, J.L., et al:  Immunoglobulin Synthesis In Vitro by Established Human Cell Lines , Science 152:1259-1261 ( (May 27) ) 1966;.
Iwakata, S.. and Grace, J.T.:  Cultivation In Vitro of Myeloblasts From Human Leukemia , New York J Med 64:2279-2282 ( (Sept) ) 1964;.
Grace, J.T.:  Recent Studies of Human Leukemia , read before the 20th Annual Symposium on Fundamental Cancer Research at the University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, March 7,1966 .
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