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Distribution of Serum Amylase in Man and Animals: Title and subTitle BreakElectrophoretic and Chromatographic Studies FREE

J. Edward Berk, MD; Ronald L. Searcy, PhD; Shinichiro Hayashi, MD; Ichiro Ujihira, MD
[+] Author Affiliations

Read before the session on gastrointestinal problems of the Fourth Multiple Discipline Research Forum during the 113th annual convention of the American Medical Association, San Francisco, June 25, 1964.

Reprint requests to 1721 Griffin Avenue, Los Angeles 31 (Dr. Berk).


From the departments of medicine, California College of Medicine, and Los Angeles County General Hospital (Drs. Berk, Ujihira, and Hayashi). Dr. Searcy is in the Department of Pathology at both institutions.


JAMA. 1965;192(5):389-393. doi:10.1001/jama.1965.03080180047012
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Many enzymes formerly viewed as single entities are now considered to be composed of a number of molecular forms termed isoenzymes.1 Although each of these isoenzymes acts on the same substrate and produces the same end product, they show certain physicochemical differences. Evidence has recently been advanced indicating that this concept of enzyme multiplicity may be extended to serum amylase.2 Such observations may have clinical significance, since serum total amylase as conventionally determined is relatively nonspecific. Serum amylase activity may be altered in such varied disorders as pancreatitis, perforated peptic ulcer, liver and biliary disturbances, peritonitis, intestinal obstruction, and ruptured ectopic pregancy.3 The level of amylase activity in the serum may also be increased by administration of opiates and analgesic drugs.4 If it should prove possible, therefore, to delineate distinctive isoenzymes of amylase and to determine their specific tissue sources, the usefulness of serum amylase in

REFERENCES

Kaplan, N.O.:  Symposium on Multiple Forms of Enzymes and Control Mechanisms: I. Multiple Forms of Enzymes , Bact Rev 27:155-169 ( (June) ) 1963;.
McGeachin, R.L., and Reynolds, J.M.:  Serological Differentiation of Amylase Isoenzymes , Ann NY Acad Sci 94:996-1003 ( (Nov 2) ) 1961;.
Webster. P.D., and Zieve, L.:  Alterations in Serum Content of Pancreatic Enzymes , New Eng J Med 267:604-607 ( (Sept 20) ) 1962;.
Wapshaw, H.:  Pancreatic Side-Effects of Morphine , Brit Med J 1:373-375 ( (Feb 14) ) 1953;.
Baker, R.W.R., and Pellegrino, C.:  Separation and Detection of Serum Enzymes by Paper Electrophoresis , Scand J Clin Lab Invest 6:94-101, 1954;.
Sober, H.A., et al:  Chromatography of Proteins: II. Fractionation of Serum Protein on Anion-Exchange Cellulose , J Amer Chem Soc 78:756-763 ( (Feb 20) ) 1956;.
Peterson, E.A., and Sober, H.A.:  Chromatography of Proteins: I. Cellulose Ion-Exchange Adsorbents , J Amer Chem Soc 78:751-755 ( (Feb 20) ) 1956;.
Blandamer, A.H., and Beechy, R.B.:  Dextran-Gel Filtration in Purification of Crustacean Alpha-Amylase , Nature 197:591-592 ( (Feb 9) ) 1963;.
Sumner, J.B.:  Dinitrosalicylic Acid: Reagent for Estimation of Sugar in Normal and Diabetic Urine , J Biol Chem 47:5-9 ( (June) ) 1921;.
Sumner, J.B., and Howell, S.F.:  Method for Determination of Saccharase Activity , J Biol Chem 108:51-54 ( (Jan) ) 1935;.
Ujihira, I., et al:  Saccharogenic Method for Estimating Electrophoretic and Chromatographic Distribution of Human Serum Amylase , Clin Chem 11:97-112 ( (Feb) ) 1965;.
McGeachin, R.L.. and Lewis, J.P.:  Electrophoretic Behavior of Serum Amylase , J Biol Chem 234:795-798 ( (June 23) ) 1959;.
Kalckar, H.M.:  Differential Spectrophotometry of Purine Compounds by Means of Specific Enzymes: III. Studies of Enzymes Purine Metabolism , J Biol Chem 167:461-475 ( (Feb) ) 1947;.
Berk, J.E., et al:  Electrophoretic Behavior of Rabbit Serum Amylase , Nature 200:572-573 ( (Nov 9) ) 1963;.
Ujihira, I.; Searcy, R.L.; and Berk, J.E.:  Characterization of Serum Amylase Activity in Normal and Pancreatectomized Dogs , Proc Soc Exp Biol Med 115:996-1000 ( (April) ) 1964;.
Berk, J.E., et al:  Chromatographic Heterogeneity of Rabbit Serum Amylase , Science 141:1182-1183 ( (Sept 20) ) 1963;.
Wilding, P.:  Use of Gel Filtration in Study of Human Amylase , Clin Chim Acta 8:918-924 ( (Nov) ) 1963;.
Dreiling, D.A.; Janowitz, N.D.; and Josephberg, L.J.:  Serum Iso-Amylases , Ann Intern Med 58:235-244 ( (Feb) ) 1963;.

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Kaplan, N.O.:  Symposium on Multiple Forms of Enzymes and Control Mechanisms: I. Multiple Forms of Enzymes , Bact Rev 27:155-169 ( (June) ) 1963;.
McGeachin, R.L., and Reynolds, J.M.:  Serological Differentiation of Amylase Isoenzymes , Ann NY Acad Sci 94:996-1003 ( (Nov 2) ) 1961;.
Webster. P.D., and Zieve, L.:  Alterations in Serum Content of Pancreatic Enzymes , New Eng J Med 267:604-607 ( (Sept 20) ) 1962;.
Wapshaw, H.:  Pancreatic Side-Effects of Morphine , Brit Med J 1:373-375 ( (Feb 14) ) 1953;.
Baker, R.W.R., and Pellegrino, C.:  Separation and Detection of Serum Enzymes by Paper Electrophoresis , Scand J Clin Lab Invest 6:94-101, 1954;.
Sober, H.A., et al:  Chromatography of Proteins: II. Fractionation of Serum Protein on Anion-Exchange Cellulose , J Amer Chem Soc 78:756-763 ( (Feb 20) ) 1956;.
Peterson, E.A., and Sober, H.A.:  Chromatography of Proteins: I. Cellulose Ion-Exchange Adsorbents , J Amer Chem Soc 78:751-755 ( (Feb 20) ) 1956;.
Blandamer, A.H., and Beechy, R.B.:  Dextran-Gel Filtration in Purification of Crustacean Alpha-Amylase , Nature 197:591-592 ( (Feb 9) ) 1963;.
Sumner, J.B.:  Dinitrosalicylic Acid: Reagent for Estimation of Sugar in Normal and Diabetic Urine , J Biol Chem 47:5-9 ( (June) ) 1921;.
Sumner, J.B., and Howell, S.F.:  Method for Determination of Saccharase Activity , J Biol Chem 108:51-54 ( (Jan) ) 1935;.
Ujihira, I., et al:  Saccharogenic Method for Estimating Electrophoretic and Chromatographic Distribution of Human Serum Amylase , Clin Chem 11:97-112 ( (Feb) ) 1965;.
McGeachin, R.L.. and Lewis, J.P.:  Electrophoretic Behavior of Serum Amylase , J Biol Chem 234:795-798 ( (June 23) ) 1959;.
Kalckar, H.M.:  Differential Spectrophotometry of Purine Compounds by Means of Specific Enzymes: III. Studies of Enzymes Purine Metabolism , J Biol Chem 167:461-475 ( (Feb) ) 1947;.
Berk, J.E., et al:  Electrophoretic Behavior of Rabbit Serum Amylase , Nature 200:572-573 ( (Nov 9) ) 1963;.
Ujihira, I.; Searcy, R.L.; and Berk, J.E.:  Characterization of Serum Amylase Activity in Normal and Pancreatectomized Dogs , Proc Soc Exp Biol Med 115:996-1000 ( (April) ) 1964;.
Berk, J.E., et al:  Chromatographic Heterogeneity of Rabbit Serum Amylase , Science 141:1182-1183 ( (Sept 20) ) 1963;.
Wilding, P.:  Use of Gel Filtration in Study of Human Amylase , Clin Chim Acta 8:918-924 ( (Nov) ) 1963;.
Dreiling, D.A.; Janowitz, N.D.; and Josephberg, L.J.:  Serum Iso-Amylases , Ann Intern Med 58:235-244 ( (Feb) ) 1963;.
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