Metabolism of the Transplanted Heart: Title and subTitle BreakEffect of Excision and Reimplantation on Myocardial ATPase and Glucose-6-Phosphate Dehydrogenase Activity FREE

Theodore Cooper, MD, PhD; Max Jellinek, PhD; Vallee L. Willman, MD; C. Rollins Hanlon, MD

[+] Author Affiliations

Read before the session on transplantation of the Fourth Multiple Discipline Research Forum during the 113th annual convention of the American Medical Association, San Francisco, June 24, 1964.

Reprint requests to 1325 S Grand Blvd, St. Louis 63104 (Dr. Cooper).

From the Department of Surgery and Center for Cardiovascular Research, St. Louis University and St. Louis University Surgical Service, Veterans Administration Hospital, St. Louis.

JAMA. 1965;191(4):304-305. doi:10.1001/jama.1965.03080040046013

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References

ABSTRACT

ABSTRACT | REFERENCES

The autotransplanted heart, while free of rejection problems peculiar to homotransplantation, presents functional abnormalities^1,2 which may extend to cardiac failure during the postoperative period.³ Nevertheless, many animals tolerate complete excision and reimplantation of the heart indefinitely.⁴ The intrinsic adaptations required of the heart during the postoperative period are incompletely understood. We have, therefore, surveyed the effect of the operation on the activities of certain myocardial enzyme systems. This report details our experience with the myocardial adenosine triphosphatase (ATPase) and glucose-6-phosphate dehydrogenase activity.

Methods Cardiac excision and reimplantation (orthotopic autotransplantation) was done in five dogs. This operation, described previously,⁵ involves transection of the aorta, pulmonary artery, venae cavae, and left atrium, after which the heart is sutured into its original position. With the animal under general anesthesia the heart is rapidly excised and frozen in dry ice after separation into atrial, ventricular, and septal components. This was

REFERENCES

ABSTRACT | REFERENCES

Willman, V.L., et al: Neural Responses Following Autotransplantation of Canine Heart , Circulation 27:713-716 ( (April) ) 1963;.

Cooper, T.; Willman, V.L.; and Hanlon, C.R.: Drug Responses of Transplanted Heart , Dis Chest 45:284-287 ( (March) ) 1964;.

Willman, V.L., et al: Mechanisms of Cardiac Failure After Excision and Reimplantation of Canine Heart , Surg Forum 13:93-94, 1962;.

Willman, V.L.; Cooper, T.; and Hanlon, C.R.: Return of Neural Responses After Autotransplantation of Heart , Amer J Physiol 207:187-189 ( (July) ) 1964;.

Willman, V.L., et al: Autotransplantation of Canine Heart , Surg Gynec Obstet 115:299-302 ( (Sept) ) 1962;.

Fiske, C.A., and Subbarow, Y.: Colorimetric Determination of Phosphorus , J Biol Chem 66:375-400 ( (Dec) ) 1925;.

Lowry, O.H., et al: Protein Measurement With Folin Phenol Reagent , J Biol Chem 193:265-275 ( (Nov) ) 1951;.

Dickens, F.; Glock, G.E.; and McLean, P.: Ciba Foundation Symposium on Regulation of Cell Metabolism , London: J. & A. Churchill, Ltd., 1959;, pp 150-187.

Willman, V.L., et al: Ventricular Function After Hypothermic Cardiac Arrest , Arch Surg 82:120-127 ( (Jan) ) 1961;.

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