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MICROBIAL SENSITIVITY TEST IN MANAGEMENT OF URINARY TRACT INFECTIONS FREE

Khurshid A. Mian, M.P.H.
[+] Author Affiliations

Ann Arbor, Mich.

Director, Department of Microbiology, St. Joseph Mercy Hospital.


JAMA. 1959;170(8):934-938. doi:10.1001/jama.1959.03010080042007
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Prompt determination of the causative organism and of its sensitiveness to various antibiotics is important in cases of infection. The test here described involves the use of a special culture medium and antibiotic disks. The medium contains whole blood and is spread, with the inoculum, on plates. After incubation, the presence of bright red zones about certain disks indicates the antibiotics to which the predominant organism is sensitive. The method has been applied in a study of 789 specimens from urinary infections, in which the organisms most frequently found were Escherichia, Proteus, and Streptococcus. Patients who had been refractory to prolonged empirical therapy responded quickly, in nearly every case, to therapy based on this testing of the causative organisms.

REFERENCES

Lind, H. E., and Swanton, E.:  Routine Bacteriologic Sensitivity Determinations in Antibiotic Treatment , Antibiotics & Chemother. 2:30-34 ( (Jan.) ) 1952;.
Vincent, J. G., and Vincent, H. W.:  Filter Paper Disc Modification of Oxford Cup Penicillin Determination , Proc. Soc. Exper. Biol. & Med. 55:162-164 ( (March) ) 1944;.
Broom, N. H.; Martineau, P. C.; and Young, D. C.:  Correlation of Results of Disk Antibiotic Sensitivity Tests and Clinical Course of 69 Patients with Acute Bacterial Meningitis , Antibiotics & Chemother. 3:409-414 ( (April) ) 1953;.
Bondi, A., Jr., and Dietz, C. C.:  Penicillin Resistant Staphylococci , Proc. Soc. Exper. Biol. & Med. 60:55-58 ( (Oct.) ) 1945;.
Bondi, A., Jr.; Dietz, C. C.; and Spaulding, E. H.:  Interference with Antibacterial Action of Streptomycin by Reducing Agents , Science 103:399-401 ( (March 29) ) 1946;.
Bondi, A., Jr.; Spaulding, E. H.; Smith, D. E.; and Dietz, C. C.:  Routine Method for Rapid Determination of Susceptibility to Penicillin and Other Antibiotics , Am. J. M. Sc. 213:221-225 ( (Feb.) ) 1947;.
Trafton, H. M., and Lind, H. E.:  Urinary Infections: Clinical and Bacteriological Cure with Terramycin , J. Urol. 69:315-319 ( (Feb.) ) 1953;.
Koch, M. L., and Bourgeois, R. W.:  Report on Further Increase in Incidence of Drug-Resistant Pathogenic Staphylococci Encountered in Hospital Laboratory , Antibiotics & Chemother. 2:229-233 ( (May) ) 1952;.
Jackson, J. L. W.; Dye, W. E.; and Mitchell, R. B.:  Use of Hemoglobin Indicator for Rapid Method of Determining Antibiotic Sensitivity of Micro-Organisms, Proceedings, Texas Branch of the Society of American Bacteriologists , Texas Rep. Biol. & Med. 12:171 ( (Spring) ) 1954;.
Levinthol, W., and Fernbach, H.:  Morphologische Studien an Influenzabacillen und des ätiologische Grippeproblem , Ztschr. Hyg. 96:456-519, 1922;.

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Lind, H. E., and Swanton, E.:  Routine Bacteriologic Sensitivity Determinations in Antibiotic Treatment , Antibiotics & Chemother. 2:30-34 ( (Jan.) ) 1952;.
Vincent, J. G., and Vincent, H. W.:  Filter Paper Disc Modification of Oxford Cup Penicillin Determination , Proc. Soc. Exper. Biol. & Med. 55:162-164 ( (March) ) 1944;.
Broom, N. H.; Martineau, P. C.; and Young, D. C.:  Correlation of Results of Disk Antibiotic Sensitivity Tests and Clinical Course of 69 Patients with Acute Bacterial Meningitis , Antibiotics & Chemother. 3:409-414 ( (April) ) 1953;.
Bondi, A., Jr., and Dietz, C. C.:  Penicillin Resistant Staphylococci , Proc. Soc. Exper. Biol. & Med. 60:55-58 ( (Oct.) ) 1945;.
Bondi, A., Jr.; Dietz, C. C.; and Spaulding, E. H.:  Interference with Antibacterial Action of Streptomycin by Reducing Agents , Science 103:399-401 ( (March 29) ) 1946;.
Bondi, A., Jr.; Spaulding, E. H.; Smith, D. E.; and Dietz, C. C.:  Routine Method for Rapid Determination of Susceptibility to Penicillin and Other Antibiotics , Am. J. M. Sc. 213:221-225 ( (Feb.) ) 1947;.
Trafton, H. M., and Lind, H. E.:  Urinary Infections: Clinical and Bacteriological Cure with Terramycin , J. Urol. 69:315-319 ( (Feb.) ) 1953;.
Koch, M. L., and Bourgeois, R. W.:  Report on Further Increase in Incidence of Drug-Resistant Pathogenic Staphylococci Encountered in Hospital Laboratory , Antibiotics & Chemother. 2:229-233 ( (May) ) 1952;.
Jackson, J. L. W.; Dye, W. E.; and Mitchell, R. B.:  Use of Hemoglobin Indicator for Rapid Method of Determining Antibiotic Sensitivity of Micro-Organisms, Proceedings, Texas Branch of the Society of American Bacteriologists , Texas Rep. Biol. & Med. 12:171 ( (Spring) ) 1954;.
Levinthol, W., and Fernbach, H.:  Morphologische Studien an Influenzabacillen und des ätiologische Grippeproblem , Ztschr. Hyg. 96:456-519, 1922;.
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