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A SUCCESSFUL METHOD FOR VACCINATION AGAINST ACUTE ANTERIOR POLIOMYELITIS: Title and subTitle BreakFURTHER REPORT FREE

JOHN A. KOLMER, M.D., LL.D., Sc.D.; GEORGE F. KLUGH, Jr., M.D.MISS ; ANNA M. RULE
[+] Author Affiliations

Aided by grants from the Daniel J. McCarthy Fund for Research in Neurology of Temple University and two anonymous donations.


JAMA. 1935;104(6):456-460. doi:10.1001/jama.1935.02760060024006
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As recently stated by Kolmer and Rule,1 it is possible to vaccinate Macacus rhesus monkeys safely and successfully against acute anterior poliomyelitis with subcutaneous and intracutaneous injections of vaccines of living but attenuated virus composed of 4 per cent suspensions of poliomyelitic monkey spinal cord in sterile 1 per cent solutions of sodium ricinoleate. All of a series of eighteen monkeys were immunized sufficiently without the slightest evidences of ill effects to protect them completely against infection following the intracerebral injection of about eighteen minimal infective doses of virus given under ether anesthesia about one month after the last dose of vaccine, the disease developing in unvaccinated controls in from five to nine days after inoculation.

Following these observations, two of us received subcutaneous injections of 0.5, 1.5 and 2 cc. of the vaccine at intervals of five days without any ill effects whatever except local reactions at the

REFERENCES

Kolmer, J. A., and Rule, Anna M.: Am. J. M. Sc. 188:510 ( (Oct.) ) 1934;.
Kolmer, J. A., and Rule, Anna M.: J. Immunology 26: 505 ( (June) ) 1934;.
We are indebted to the William Merrell Company for a generous supply of this substance (patent 1621118).
Larson, W. P., and others: Proc. Soc. Exper. Biol. & Med. 21: 278, 1924;
22, 194, 1924.
Larson, W. P., Proc. Soc. Exper. Biol. & Med. 23:497 ( (March) ) 1926;.
Kolmer, J. A.; Rule, Anna M., and Madden, Bernard: J. Lab. & Clin. Med. 19:972 ( (June) ) 1934;.
McKinley, J. C., and Larson, W. P.: Proc. Soc. Exper. Biol. & Med. 24:297 ( (Jan.) ) 1927;.
Flexner, Simon, and Lewis, P. A.:  Experimental Poliomyelitis in Monkeys , J. A. M. A. 54: 1780 ( (May 28) ) 1910;.
Aycock, W. L., and Kagan, J. R.: J. Immunology 14:85 ( (Aug.) ) 1927;.
Stewart, F. W., and Rhoads, C. P.: J. Exper. Med. 48:959 ( (June) ) 1929;.
Rhoads, C. P., J. Exper. Med. 51:1 ( (Jan.) ) 1930;
53:115 (Jan.) 1931.
Kolmer, J. A.:  Susceptibility and Immunity in Relation to Vaccination in Acute Anterior Poliomyelitis , Am. J. M. Sc. , to be published.
Brodie, Maurice: J. Immunology 27:395 ( (Oct.) ) 1934;.
Flexner, Simon, and Amoss, H. L.: J. Exper. Med. 25:499 ( (April) ) 1917;.
Amoss, H. L.: South. M. J. 23: 18 ( (Jan.) ) 1930;.
Leiner, C., and von Wiesner, R.: Wien. klin. Wchnschr. 23:323, 1910;.
Howitt, Beatrice F.: J. Infect. Dis. 51:565 ( (Nov.) -Dec.) 1932;.
Kolmer, J. A., and Rule, Anna M.: J. Immunology , to be published.
Kolmer, J. A.; Klugh, George, Jr., and Rule, Anna M.: J. Immunology , to be published.
Kolmer, J. A., and Rule, Anna M.: J. Immunology , to be published.

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Kolmer, J. A., and Rule, Anna M.: Am. J. M. Sc. 188:510 ( (Oct.) ) 1934;.
Kolmer, J. A., and Rule, Anna M.: J. Immunology 26: 505 ( (June) ) 1934;.
We are indebted to the William Merrell Company for a generous supply of this substance (patent 1621118).
Larson, W. P., and others: Proc. Soc. Exper. Biol. & Med. 21: 278, 1924;
22, 194, 1924.
Larson, W. P., Proc. Soc. Exper. Biol. & Med. 23:497 ( (March) ) 1926;.
Kolmer, J. A.; Rule, Anna M., and Madden, Bernard: J. Lab. & Clin. Med. 19:972 ( (June) ) 1934;.
McKinley, J. C., and Larson, W. P.: Proc. Soc. Exper. Biol. & Med. 24:297 ( (Jan.) ) 1927;.
Flexner, Simon, and Lewis, P. A.:  Experimental Poliomyelitis in Monkeys , J. A. M. A. 54: 1780 ( (May 28) ) 1910;.
Aycock, W. L., and Kagan, J. R.: J. Immunology 14:85 ( (Aug.) ) 1927;.
Stewart, F. W., and Rhoads, C. P.: J. Exper. Med. 48:959 ( (June) ) 1929;.
Rhoads, C. P., J. Exper. Med. 51:1 ( (Jan.) ) 1930;
53:115 (Jan.) 1931.
Kolmer, J. A.:  Susceptibility and Immunity in Relation to Vaccination in Acute Anterior Poliomyelitis , Am. J. M. Sc. , to be published.
Brodie, Maurice: J. Immunology 27:395 ( (Oct.) ) 1934;.
Flexner, Simon, and Amoss, H. L.: J. Exper. Med. 25:499 ( (April) ) 1917;.
Amoss, H. L.: South. M. J. 23: 18 ( (Jan.) ) 1930;.
Leiner, C., and von Wiesner, R.: Wien. klin. Wchnschr. 23:323, 1910;.
Howitt, Beatrice F.: J. Infect. Dis. 51:565 ( (Nov.) -Dec.) 1932;.
Kolmer, J. A., and Rule, Anna M.: J. Immunology , to be published.
Kolmer, J. A.; Klugh, George, Jr., and Rule, Anna M.: J. Immunology , to be published.
Kolmer, J. A., and Rule, Anna M.: J. Immunology , to be published.
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