The intent of the editors of this book (originally published as a supplement to volume 14 of Laboratory Investigation) is to bring together in one volume papers on topics from all aspects of electron microscopy that previously were available only from widely scattered sources. Papers by various authors are arranged in a more or less logical sequence starting with image formation and progressing to the final electron micrograph and its quantitative evaluation.
The range of topics dealt with is wide, encompassing both biological and physical problems in electron microscopy. Many of the papers dealing with the physical aspects of electron microscopy are theoretical and, thus, of relatively little value to biologists, whereas others give practical information on such things as determination of magnification, reduction of contamination and the like that will be generally useful. However, the problems (such as fixation, dehydration, embedding and sectioning of tissues) that face the biologist