Chromosomal Changes in Leukocytes of Patients Receiving Irradiation Therapy

Shields Warren, MD; Lorraine Meisner, MA
JAMA. 1965;193(5):351-358. doi:10.1001/jama.1965.03090050027008.
Text Size: A A A
Published online

Human chromosomes may be readily studied by in vitro culture of leukocytes obtained from peripheral blood1 (or even from bone marrow) to which phytohemagglutinin2 has been added. The dividing cells are lymphocytes and perhaps monocytes,3,4 and their chromosomal complement is representative of that of other body cells.4 Cells of skin and fetal and other tissues, including neoplasms, can be grown in tissue culture and studied in mitotic metaphase, but these preparations are not as readily suitable for study of chromosomes. Tjio and Levan5 in 1956 greatly stimulated interest in chromosomes by their presentation of the human karyotype.

The purpose of this study is to explore the effects of in vivo x-radiation on the chromosomes of normal human somatic cells. This has been done by analyzing cultured leukocytes from cancer patients who have had much hemopoietic tissue exposed in the course of x-ray therapy. Taking samples


Sign In to Access Full Content

Don't have Access?

Register and get free email Table of Contents alerts, saved searches, PowerPoint downloads, CME quizzes, and more

Subscribe for full-text access to content from 1998 forward and a host of useful features

Activate your current subscription (AMA members and current subscribers)

Purchase Online Access to this article for 24 hours

First Page Preview

View Large
First page PDF preview




Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).


Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

Related Content

Customize your page view by dragging & repositioning the boxes below.