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Isolation and Storage of Artificially Oxygenated Mammalian Hearts

Jerome F. Levy, MD; Harvey R. Bernard, MD; William F. Monafo Jr., MD
JAMA. 1965;191(12):1006-1008. doi:10.1001/jama.1965.03080120040010.
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A satisfactory method by which viable and uninjured mammalian hearts may be removed and stored is important to the study of cardiac homotransplantation. This report describes a method of cardiac isolation and storage.

Methods  All animals were anesthetized with pentobarbital (30 mg/kg) and ventilated by an automatic respirator using room air. Series 1 consisted of 24 experiments using 48 mongrel dogs (10 to 20 kg) to study the technical aspects of cardiac isolation, storage, and parallel transplantation. Series 2 consisted of 16 experiments using 16 mongrel dogs (10 to 23 kg) to study improvements in the methods for isolation and storage of the heart.

Series 1.—  A bilateral anterior thoracotomy was performed and both sides of the heart of the donor animal were perfused with a variety of cold (4 C) electrolyte containing solutions after ligation of the vena cava. All vessels were then ligated and divided and the heart


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