Isolation and Storage of Artificially Oxygenated Mammalian Hearts

Jerome F. Levy, MD; Harvey R. Bernard, MD; William F. Monafo Jr., MD
JAMA. 1965;191(12):1006-1008. doi:10.1001/jama.1965.03080120040010.
Text Size: A A A
Published online

A satisfactory method by which viable and uninjured mammalian hearts may be removed and stored is important to the study of cardiac homotransplantation. This report describes a method of cardiac isolation and storage.

Methods  All animals were anesthetized with pentobarbital (30 mg/kg) and ventilated by an automatic respirator using room air. Series 1 consisted of 24 experiments using 48 mongrel dogs (10 to 20 kg) to study the technical aspects of cardiac isolation, storage, and parallel transplantation. Series 2 consisted of 16 experiments using 16 mongrel dogs (10 to 23 kg) to study improvements in the methods for isolation and storage of the heart.

Series 1.—  A bilateral anterior thoracotomy was performed and both sides of the heart of the donor animal were perfused with a variety of cold (4 C) electrolyte containing solutions after ligation of the vena cava. All vessels were then ligated and divided and the heart


Sign In to Access Full Content

Don't have Access?

Register and get free email Table of Contents alerts, saved searches, PowerPoint downloads, CME quizzes, and more

Subscribe for full-text access to content from 1998 forward and a host of useful features

Activate your current subscription (AMA members and current subscribers)

Purchase Online Access to this article for 24 hours

First Page Preview

View Large
First page PDF preview




Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).


Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

Related Content

Customize your page view by dragging & repositioning the boxes below.