On March 9, 2010, two stray dogs found by a sheriff's deputy in Marshall County, Minnesota, were brought to an animal shelter in Grand Forks, North Dakota. Marshall County is a rural area of Minnesota, and Grand Forks offers the closest animal shelter. In accordance with animal shelter protocol and city ordinance, the dogs were isolated from other animals in the shelter for 5 days. During this time, the dogs were observed for signs of disease or behavioral abnormalities. Dog A was fearful of shelter staff members and dependent on dog B, which was dominant, aggressive, and larger than dog A. On March 15, after the 5 days of isolation, the two dogs were transferred to the area holding the general shelter population and made available for adoption. Because of its dominant and aggressive temperament, however, dog B was deemed unsuitable for adoption and euthanized on March 19. On March 20, dog A was placed with a foster family in North Dakota. Five days later, the dog was vomiting and had loss of balance. On March 27, the family returned the dog to the shelter, where it was examined by a veterinarian, who noted hyperesthesia, tremors, ataxia, and dilated pupils. Because the differential diagnosis included canine distemper and rabies, the dog was euthanized the same day, and the brain was sent to the state veterinary diagnostic laboratory for testing. Three days later, the laboratory reported that a fluorescent antibody test was positive for rabies virus. CDC confirmed the result and characterized the virus as a North Central skunk rabies virus variant.