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ARTIFICIAL SUSCEPTIBILITY TOWARD THE BACILLUS OF SOFT CHANCRE.

JAMA. 1902;XXXVIII(4):253. doi:10.1001/jama.1902.02480040039005.
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In 1889 Ducrey reported that in successive chancroidal ulcerations produced by inoculations of human beings with the chancroidal pus there occurs constantly the same bacillus. Since then the etiologic relationship of this bacillus has been confirmed by Krefting, Colombini and others. The bacillus has been cultivated upon various media, especially serum-agar, and typical ulcerations have been produced in human beings by inoculations of pure cultures of the bacillus. Recently Himmel1 has shown that coagulated blood of guinea-pigs, allowed to stand for a time or heated to 55 C. for half an hour, constitutes an excellent nutrient medium for the bacillus of soft chancre. He recommends cultures upon this medium as an auxiliary method of differential diagnosis in doubtful clinical cases. As was the case with previous investigators, Himmel also failed to obtain positive results by injections into animals of pure cultures of the bacillus. In guinea-pigs the bacilli, injected subcutaneously

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