JAMA. 1901;XXXVI(26):1832. doi:10.1001/jama.1901.02470260010005.
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The determination of the nature of traces of blood for forensic purposes is a very difficult problem, which often can not be cleared up sufficiently to afford definite aid to the administration of law and justice. Soon after the discovery, by Bordet, of cytotoxins produced by injecting the blood of an animal into another animal of a different species, it was found that hemolytic serum also has a precipitating action. It has been shown by Nolf that the precipitation develops as the result of the injection of serum without admixture of red corpuscles. Like the hemolytic action, the precipitating property is also strictly specific, becoming evident only when the serum is mixed with the blood of the species that furnished the blood for injection. Deutsch1 found that prepared serum has the same—both hemolytic and precipitating—action on dried as on fresh blood, and thus the idea developed that serum diagnosis


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