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CHOLESTERINIZED ANTIGENS

LOYD THOMPSON, Ph.B., M.D.
JAMA. 1914;LXII(19):1458-1459. doi:10.1001/jama.1914.02560440014004.
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In a recent article,1 Thomas and Ivy decry the use of cholesterinized antigens in the Wassermann reaction, asserting that many positive results are obtained by them in non-syphilitic cases.

Since Dec. 1, 1913, I have been using in my Wassermann work an antigen composed of human heart-extract plus 0.4 per cent. cholesterin as advocated by Walker and Swift.2 Some of this was very kindly furnished me by Dr. Ralph W. Webster, while the remainder was prepared in my own laboratory.

In all I have used the cholesterinized antigen in 356 tests. While this number is somewhat small from which to draw definite conclusions, it is larger than that of Thomas and Ivy, and my results have been so uniformly satisfactory that I unqualifiedly endorse the use of this antigen, even over the heads of such authorities.

In beginning the use of the cholesterinized antigen I felt that a

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