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R. O. LeBARON, B. Sc., M.D.
JAMA. 1907;XLIX(25):2084. doi:10.1001/jama.1907.25320250036002.
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The staining solution is made by saturating methyl alcohol (97 per cent.) with fuchsin and adding 5 per cent. of carbolic acid. This fluid will stain the tubercle bacilli in sputum in four minutes without employing heat. As the stain is dissolved in methyl alcohol, preliminary fixation is really unnecessary, but I have found that, at least with some sputums, a smoother field is obtained by a light fixation in the flame. (The sputums were all incubated for from twelve to twenty-four hours.) The specimen is then covered with the stain, which is allowed to remain at least four, but not more that five, minutes, timed by the watch; washed thoroughly in water; decolorized (dilute nitric acid and 60 per cent. alcohol used separately), and counterstained as ordinarily. The counterstain should be rather light. The tubercle bacilli are stained deeply and the field is unusually clear.


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