Rochester, N. Y., Oct. 16, 1907.
To the Editor:
—The vagina normally contains myriads of organisms which are not incompatible with health. Since they are non-irritating, they have little power of penetrating the epithelium, hence they are for the most part to be found on the surface epithelial cells. When a poisonous organism, as the gonococcus, is implanted in the vagina, the profuse inflammatory discharge therefrom offers a first-rate medium for the growth of the numerous harmless secondary germs with the result that the gonococcus is often overshadowed and the microscopic diagnosis obscured. This is often of great importance, both clinically and medicolegally. The usual method of differentiation has been by contrast staining, but as a rule this is so unsatisfactory as to have but limited value. To obviate this difficulty I have employed the following procedure with most excellent results. Advantage is taken of the fact that the gonococci