In 1899, in association with E. M. Ranck, I published a paper in the Journal of Comparative Medicine and Veterinary Archives entitled "Tetanus Toxin and Antitoxin." In addition to general facts regarding tetanus, the tetanus bacillus and the toxin and antitoxin, there were recorded some practical experiences with the antitoxin as a prophylactic, which, probably because of the nature of the rest of the paper, escaped the attention they deserved.
The observations to which I again call attention were made in one of the large antitoxin factories of this country on a number of horses somewhat exceeding 800, and continuing over a period of nearly four years. These 800 horses were used for immunization experiments in manufacturing different therapeutic serums, and were constantly being injected and bled at frequent intervals, and occasionally suffered from local lesions consequent upon the irritative action of the injected toxins.
At the time the laboratory