This paper is based on observations made on 200 patients examined serologically for evidences of syphilis. The work done has covered a period of one and one-half years. The material was obtained from the medical wards of St. Margaret's Hospital. The data given represent our effort to develop a Wassermann technic which would check up in a larger percentage of cases with the clinical diagnosis of syphilis and which would enable us to attach some significance to the negative Wassermann reaction.
Our method consists essentially in the use of graded increasing amounts of blood serum and spinal fluid, each serum being tested in the following amounts: 0.1 c.c., 0.3 c.c., 0.5 c.c. and 1 c.c., while amounts of spinal fluid as high as 10 c.c. are used in a single test.
Our investigation shows that as much as 1 c.c. of serum may be safely used, and that a negative