The first studies of the hemolytic action of serpents' venoms seem to have been made by Stevens and Myers,1 who found that when cobra venom was added to shed blood, in vitro, destruction of the corpuscles (hemolysis) and retardation of coagulation took place. They also found the hemolytic power of the venom inhibited by antivenomous serum.
Myers2 later studied the hemolytic substance—"cobralysine"—of the venom and found it destroyed by heat. During these studies he found that the susceptibility of the erythrocytes to cobralysine in vitro bore no relation to the susceptibilty of the animal to subcutaneous intoxication by venom.
Stephens3 found that the hemolytic constituents of different venoms were not identical.
Myers4 made further studies of venom hemolysins and serum antihemolysins.
Mitchell and Flexner5 and Flexner and Noguchi6 have further studied the phenomena of agglutination, hemolysis, leucolysis, and the germicidal activity of the blood serum. They found that the