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February 27, 1909, Vol LII, No. 9 >
ARTICLE | February 27, 1909

ACCIDENTAL LABORATORY INFECTION WITH DIPHTHERIA BACILLUS

M. L. HOLM, M.D.
JAMA. 1909;LII(9):698-699. doi:10.1001/jama.1909.25420350024002a.
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ABSTRACT

History.  —On December 2 I received at the laboratory a very thin homeopathic vial containing a suspected diphtheritic membrane for examination. In attempting to remove the cork the vial was broken and a piece of glass was driven into my left index finger. The wound was allowed to bleed freely for several minutes, then carefully cleansed with a 10 per cent. solution of formalin and closed with celloidin. The wound in the finger showed no sign of infection before the morning of the second day, when a grayish yellow border was observed around the margin. The wound was opened and found to contain several drops of thick grayish brown fluid, which on microscopic examination was found to consist largely of polymorphonuclear leucocytes and some blood. Numerous B. diphtheriæ were present, all of which were found inside the leucocytes, there being from two to five such leucocytes to the field, each

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