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Use of Optical Immunoassay to Diagnose Streptococcal Pharyngitis-Reply

Michael A. Gerber, MD; Robert R. Tanz, MD; Stanford T. Shulman, MD; Edward L. Kaplan, MD
JAMA. 1997;278(1):23-24. doi:10.1001/jama.1997.03550010037029.
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In Reply.  —Dr Dale and colleagues suggest that the relatively high sensitivity of OIA compared with BAP culture observed in our investigation was more a reflection of the poor quality of BAP culture than of the high quality of OIA. We do not believe this to be the case.The pediatricians' offices that participated in our study were selected because many of these sites had participated in similar research projects, and all had office laboratories that were certified under the Clinical Laboratories Improvement Act. In addition, all received instruction in the proper performance of BAP cultures prior to the study. Therefore, it is reasonable to assume that the quality of BAP culturing in these offices was considerably better than that of the typical physician's office. With placement of the bacitracin disk on the primary BAP culture, as was done in our study, only about half of the specimens with GABHS can be


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