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Reevaluation of Serum-Plasma Differences in Total Cholesterol Concentration

Teresa Cloey; Paul S. Bachorik, PhD; Diane Becker, ScD, MPH; Cheryl Finney, MPH; David Lowry, MA, MPH; William Sigmund, MD
JAMA. 1990;263(20):2788-2789. doi:10.1001/jama.1990.03440200092029.
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We measured total cholesterol levels in serum and disodium ethylenediaminetetraacetate (EDTA) plasma samples obtained from 84 healthy medical students during their entrance physical examinations and 48 adults from a cholesterol screening program who were resampled because they had initial values of 5.17 mmol/L (200 mg/dL) or higher. Plasma cholesterol concentrations were 4.7% lower than those in serum samples. The plasma and serum values were highly correlated (r=.994), however, suggesting that measurements in EDTA plasma can be converted readily to equivalent serum concentrations. Thus, the negative bias in EDTA plasma was greater than the 3% value cited in the National Cholesterol Education Program Guidelines, probably because the amount of EDTA now provided in evacuated blood collection tubes is 50% greater than in those used when the 3% value was established. These findings are relevant to the interpretation of both cholesterol screening measurements and follow-up lipoprotein analyses.

(JAMA. 1990;263:2788-2789)


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