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September 22, 1989, Vol 262, No. 12 >
ARTICLE | September 22, 1989

Probes for Identifying Listeria

Atin R. Datta, PhD
JAMA. 1989;262(12):1629. doi:10.1001/jama.1989.03430120079016.
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To the Editor.—  I read with great interest the review article by Drs Gellin and Broome.1 The article is well written and provides an in-depth study of the problem of listeriosis. However, there is one element in the article that needs further clarification. The authors state that both monoclonal antibody— and DNA probe—based tests identify Listeria at the genus level. While this is true for the antibody-based assay, it is not true for the probe, which has been developed by my colleagues and myself at the Food and Drug Administration. The natural DNA probe that the authors quote2 in their article has been developed from a putative β-hemolysin gene fragment of Listeria monocytogenes cloned by Robert K. Flamm, PhD.3 To date, our results have shown that this probe hybridizes only with L monocytogenes strains and does not react with any other Listeria and non-Listeria strains in

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