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Women who had had negative serologic tests for rubella within 1 year before vaccination were considered susceptible at vaccination.
Since July 1985, the CDC laboratory has tested for rubella-specific IgM antibody using an indirect enzyme immunosorbent assay (EIA) with an enzyme-conjugated antihuman IgM serum. An IgM index is calculated for each serum specimen using a known low-positive IgM serum specimen as a reference standard. An IgM index greater than or equal to 1.0 is considered positive, with increasing values indicating increasing antibody levels. Before July 1985, the CDC laboratory performed sucrose density gradient centrifugation and hemagglutination-inhibition (HI) tests for rubella- specific IgM.
Persons are considered succeptible unless they can present documentation of laboratory evidence of immunity and/or documentation of adequate immunizationrubella vaccine on or after their first birthdbirthday.13