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Lactic Dehydrogenase Electrophoresis

Albert W. Opher; Charles S. Collier; Joseph M. Miller, MD
JAMA. 1968;206(2):378. doi:10.1001/jama.1968.03150020094035.
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To the Editor:—  A recent informative laboratory report (205:294, 1968) reviews the technique of agargel electrophoresis for lactic dehydrogenase isoenzymes. The authors state that the method is simple, so that it can be used in the routine laboratory, requires only about one hour for separation of the isoenzymes, and yields sharp and distinct patterns.The objection of the authors to the use of cellulose acetate is that a delicate "sandwiching" technique is needed to develop the isoenzyme pattern. This disapproval is not valid for polyacetate strips can be successfully used in a direct manner (Clin Chem12:308, 1966). After electrophoresis at 200 volts for 1.5 hours, the strips are placed in a solution containing a substrate for the enzyme and a color developer which shows the zones of isoenzyme activity as dark-purple formazan bands. The overall reaction involves the oxidation of sodium lactate and the reduction of tetra-nitroblue


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