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Aneuploidy in Cultured Amniotic Cells

J. C. Lee, MD, PhD; Richard P. Bendel; Doris C. Brooker, MD
JAMA. 1972;219(9):1211. doi:10.1001/jama.1972.03190350047017.
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To the Editor—  Although aneuploidy is not a common occurrence in cultured cells,1 sometimes it may cause difficulty in interpretation. The following case is representative: A 20-year-old white woman, para 1, gravida 2, had an amniocentesis performed at the 24th week of gestation. Eighteen months prior to this pregnancy, she had been delivered of a boy with trisomic Down's syndrome. Neither she nor her husband had a family history of genetic anomalies. Amniotic cells were cultured in 35-mm petri dishes (Falcon plastic) using HAM's F-10 nutrient medium supplemented with 15% fetal calf serum and antibiotics.2 The cells were harvested and chromosomal analysis performed 22 days after the initial culture. Fifty mitoses were analyzed. Of these, 46 cells showed normal 46-XY karyotypes. In one of the two cells that contained 45 chromosomes, there was one missing G-group chromosome. The other lacked one F-group chromosome. One cell which contained 44

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