Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address Please contact the publisher to request reinstatement.
We're unable to sign you in at this time. Please try again in a few minutes.
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Article |

A Simple and Reliable Test for Sickling

Paul Heller, MD; Vincent Yakulis
JAMA. 1968;203(11):990. doi:10.1001/jama.1968.03140110082028.
Text Size: A A A
Published online


To the Editor:—  The paper of Schneider, Alperin, and Lehmann (202:419, 1967) pointed out the occasional difficulties and pitfalls of the standard sickling test performed in the clinical laboratory. A much simpler and more reliable test for the clinical laboratory is the slideelution test which we developed a few years ago (Blood24:198, 1964). This test is based on the fact that hemoglobin S is insoluble in high molar phosphate buffer. Blood smears are prepared from capillary blood in the same way as for staining, but the unstained slides are placed into Coplin jars filled with 50 ml of 2.4 O M phosphate buffer (20.9 g/100 cc of K2HPO4 and 16.3 g/100 cc of KH2PO4; the solution is stable and we have used it for longer than three months after preparation). Just prior to the placement of the slide a small amount


Sign in

Purchase Options

• Buy this article
• Subscribe to the journal
• Rent this article ?




Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.


Some tools below are only available to our subscribers or users with an online account.

0 Citations

Sign in

Purchase Options

• Buy this article
• Subscribe to the journal
• Rent this article ?

Related Content

Customize your page view by dragging & repositioning the boxes below.