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Histological Techniques for Electron Microscopy

Frank W. Fitch, M.D.
JAMA. 1961;176(6):556. doi:10.1001/jama.1961.03040190078039.
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As pointed out by the author, the most efficient, effective, and stimulating way to learn the techniques of electron microscopy is to serve an apprenticeship in an established "teaching laboratory." For many, however, this is impossible; and it is they who will find this practical handbook invaluable. After discussion of the practical problems of organization and management of an electron microscope laboratory, the technical manipulations of preparing tissue for microscopy are detailed. The various methods of fixation and embedding are evaluated. The author prefers methacrylate embedding; however, it seems that epoxy resins will have wider use as embedding agents in the future and probably deserve a greater coverage. Sectioning and section mounting as well as microscopy are considered at length. A practical discussion of photography is especially helpful. The final chapter deals with shadow-casting, replication, and other alternate methods of specimen preparation.

This volume will be especially valuable for those


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