This book reflects a felicitous combination of basic biochemical principles and practical techniques. The first chapters deal with such aspects as cold knife and microtome methods, freeze-drying, and the chemistry of fixation. The major groups of biochemical components—proteins, nucleic acids, carbohydrates, and lipids—are considered in chapters 5 to 12. Each chapter contains a brief general survey of the chemistry of the group of compounds discussed. This is followed by a consideration of the chemical reactions involved in the staining reactions and by a critical evaluation of various methods that have been proposed in each connection. For each of these chapters, as for others in the book, a correspondingly numbered appendix gives the details of the methods recommended by the author.
Chapters 12 to 22 are devoted to the histochemistry of enzymes. The general principles are outlined in the first of these chapters. The author stresses the necessity for understanding the