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George N. Papanicolaou, M.D., Ph. D.; Emma Lou Bridges
JAMA. 1957;164(12):1330-1331. doi:10.1001/jama.1957.62980120008007c.
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As exfoliative cytology has become more widely used, the need for an adequate technique for mailing fresh, unstained smears to a cytology laboratory without exposing them to drying, which is known to distort the structural characteristics of the cells and their nuclei, has been more keenly felt.

Several techniques for mailing fresh, unstained smears have already been suggested. Ayre's glycerin method1 has found, thus far, the widest application. Other methods proposed more recently are the propylene glycol and the rehydration techniques.2

The method presented in this report was described by one of us (G. N. P.) in 1954.3 Since then it has been intermittently used in our laboratory with gratifying results. It provides a preliminary fixation of the smears and the formation of a film which protects the smear surface during mailing. The staining reaction of the cells shows no appreciable change.

In recent months, the technical


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