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JAMA. 1948;137(9):790. doi:10.1001/jama.1948.02890430032010.
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Adopting a technic previously used by them for a study of the immunologic depression due to thiamine, riboflavin and vitamin A deficiencies.1 Guggenheim and Beuchler2 of the Department of Bacteriology, Hebrew University, Jerusalem, placed groups of young rats on well balanced diets containing varying amounts of different animal or vegetable proteins. After keeping the rats on these diets for four weeks, tests for the bactericidal and phagocytic properties of their peritoneal fluids were made, with parallel determinations of their change in body weight. In a typical experiment, four diets containing 18, 9, 6 and 3 per cent casein, respectively, were used. At the end of four weeks each rat was given intraabdominal injection of 0.5 cc. of a twenty-four hour broth culture of Salmonella typhi murium. Two hours later peritoneal fluid was withdrawn and the number of leukocytes, phagocytes and extracellular and intracellular bacteria per microscopic field was


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