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A TISSUE SUBSTRATE MICROCULTURE FOR TUBERCLE BACILLI

H. J. CORPER, M.D.
JAMA. 1932;99(16):1315-1320. doi:10.1001/jama.1932.02740680011003.
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The discovery of scientific facts of practical significance has not always led to their immediate practical extensive application but has awaited some simple modification of methods which could be quickly and easily performed by the majority of practitioners. The clinician has been aware of the limited value of the microscopic examination of the stained smear for acidfast bacilli, and yet the simplicity and speed of this method has almost restricted the examination for tubercle bacilli to its use by the physician. He has had to be content with this because guinea-pigs and culture mediums required facilities which were hardly justified for his daily routine. The fact that he usually obtained a single specimen, and that especially only in the advanced case could he be certain to find acid-fast bacilli by this method, could hardly concern him. He was aware that this method1 required as many as 100,000 acid-fast bacilli

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