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JAMA. 1949;141(12):848. doi:10.1001/jama.1949.02910120036015.
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A HYPOTHESIS OF THE PRODUCTION OF DENTAL CARIES  It is assumed that lactic acid results from reaction between lactobacilli in the mouth and carbohydrate material packed in the grooves in the biting surfaces of the teeth. The acid thus produced attacks the enamel and causes caries. Pincus1 points out that the grooves of molar teeth are shallow cusps on the biting surface with extension far into the tooth. Such extensions are narrow and deep. The protein filling the groove may remain undisturbed, but the groove protein, the enamel protein and the dentine protein all contain combined sulfuric acid. Many bacteria, including strains of gram-negative bacilli in the carious material, can form from sulfatase, an enzyme which acts on a polysaccharide in these proteins releasing sulfuric acid. As yet the enzyme has not been shown to attack the dentine or enamel proteins but has been

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