For more than two years Menegaux and Odiette have been studying the reaction of bone to various metals, from the biologic point of view, because they have found that rarefying osteitis, subsequent pathologic fracture, and other clinical failures resulted from the use of metal fixation devices in fractures. After performing more than two thousand tissue culture experiments and more than a hundred operations on animals, they feel qualified to declare which metals are desirable for use in bone.
Tissue cultures of fibroblasts and osteoblasts from the chick embryo and fibroblasts from the human embryo (cases of extrauterine pregnancy) were subjected to the effects of forty different metals and alloys. Careful microscopic studies of the cultures and quantitative analyses were made. In the animal experiments, metal disks were placed under the periosteum, wire loops were placed in and around the bone, and Lane plates and screws of different metals were applied.