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TESTS FOR INFECTIOUS HEPATITIS

O. W. Haffke Captain, M. C.
JAMA. 1945;129(9):647. doi:10.1001/jama.1945.02860430063022.
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ABSTRACT

To the Editor:—  In regard to the methylene blue test used in infectious hepatitis, as reported in recent issues of The Journal, I have made some of the following observations:One patient with early clinical jaundice of the infectious hepatitis type did not have a positive methylene blue test (5 drops of methylene blue with a still green coloration of the urine).Patients recovering from infectious hepatitis who showed a negative methylene blue test still had bilirubin in the urine.Patients not suffering from infectious hepatitis will show a green coloration of the urine with as high as 3 drops of methylene blue before the blue coloration is noted. Normally one does not have bilirubin in the urine.I have made serial dilutions of an atabrine solution (yellow) and have demonstrated a progressive increase in the number of drops of methylene blue needed to change the solution from green to

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