In this paper it is proposed to give completely the results of the survey for intestinal protozoa in food handlers conducted during the past three years in some of the hospitals, hotels, restaurants, clubs and lunch rooms of San Francisco. A preliminary report1 has been published during the progress of this investigation. A total of 1,072 employees of public dining rooms and kitchens was examined and it was found that twenty-two, or 2.04 per cent, were infected with Endamoeba histolytica.
There were no fresh examinations of the stool specimens made, as it was thought that the most efficient method for disclosing protozoan infections with economy of time and labor was to make the fecal smears on the premises, directly after the passage of the stool, immersing them immediately in the fixing fluid. The smears, then brought to the laboratory, were stained by the iron alum-hematoxylin method and subsequently examined. Every