Recovery of the virus from the cerebrospinal fluid of patients with encephalitis has rarely been reported. There are only 2 reports of this in the medical literature.1
During the epidemic of western equine encephalitis in Manitoba in July and August 1941, positive results were obtained with the spinal fluid from a 6 weeks old baby admitted to the hospital on August 19 with fever and convulsions which had become apparent the previous day. Lumbar puncture was done on admission. The fluid was slightly cloudy and contained 998 cells per cubic millimeter, 80 per cent of which were polymorphonuclears. There was a decided increase in globulin content. Organisms were not found on smear or culture.
On August 20, guinea pigs 1 and 2, each weighing approximately 300 Gm., were each injected intracerebrally with 0.2 cc. of spinal fluid from this patient. Guinea pig 2 remained well. On August 26 the