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A MECHANICAL DEVICE THAT PRODUCES UNIFORM DISPERSION OF BLOOD CELLS IN THE DILUTING PIPET

W. Ray Bryan, Ph.D.; W. E. Garrey, M.D.
JAMA. 1934;103(14):1059-1060. doi:10.1001/jama.1934.72750400001009.
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A study of the reports of leukocyte counts reveals contradictions and discrepancies that are clearly the result of faulty technic. A systematic study has revealed that a significant source of error is the failure to obtain a uniform dispersion of blood cells in the diluting pipet, which is reflected in variations of the numbers of cells as well as in their uneven distribution over the field of the counting chamber. Errors of surprising magnitude in the widely used manual method of shaking the diluting pipet in one plane were revealed in our study, and they were even more marked with certain mechanical shakers known to have been used in a number of research laboratories. The extent of the errors due to the use of a mechanical shaker is illustrated in table 1, which records typical results when as many successive counts as possible (nineteen) were made from the contents of

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