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CLINICAL SIGNIFICANCE OF HORMONE ASSAYS

S. CHARLES FREED, M.D.
JAMA. 1941;117(2):103-110. doi:10.1001/jama.1941.72820280006007.
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Studies of the hormonal content of urine, blood and tissues are of significant value only when considered in relation to the clinical history, physical findings and other laboratory data of the patient. There are few tests which when considered apart from other evidence can be used safely for diagnostic purposes. In the past several years intensive investigations have pointed to the variability and complexity of tests performed for the purpose of diagnosing endocrine conditions. The results are complicated by the fact that the different laboratories vary in their technics and that there are factors of many sorts over which little control can be exercised and which interfere with the accumulation of uniform data. Definite progress has been made, however, by the use of biochemical procedures, in determining the quantities of certain hormonal substances. This advancement has been especially marked in assaying the numerous steroids of the ovary, testis and adrenal

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