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JAMA. 1927;88(6):415-416. doi:10.1001/jama.1927.02680320051020.
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ABSTRACT

Serotherapy in Botulism  Researches have been made on the botulinus toxin, on the varieties of botulinus, and on therapeutic inoculation with the corresponding antitoxin. Type B of Bacillus botulinus appears to be characteristic of Europe and Asia, and type A of America. But the importation of canned foods of various origin makes it possible to encounter the two types almost anywhere. Type B is killed by heating for fifteen minutes at a temperature of 85 C. Type A resists a temperature of 100 C. for four hours. In experiments, a chicken was found to be extremely susceptible to type A, and only to type A. A few hours after ingestion of canned food infected with type A, paralysis develops which begins in the muscles of the neck, where the symptoms are plainly visible, and then becomes generalized over the whole body. The cat also reacts readily, manifesting very early dilatation

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